Donor selection for adoptive immunotherapy with NK cells in AML patients: Comparison between analysis of lytic NK cell clones and phenotypical identification of alloreactive NK cell repertoire.

NK alloreactivity acute myeloid leukemia (AML) adoptive immunotherapy donor selection human leucocyte antigen (HLA) killer immunoglobulin-like receptors (KIR) natural killer cells (NK cells)

Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2023
Historique:
received: 29 11 2022
accepted: 31 01 2023
entrez: 3 3 2023
pubmed: 4 3 2023
medline: 7 3 2023
Statut: epublish

Résumé

Natural killer (NK) cell-based adoptive immunotherapy in leukemia patients is an emerging field of interest based on clinical evidence of efficacy and safety. Elderly acute myeloid leukemia (AML) patients have been successfully treated with NK cells from HLA-haploidentical donors, especially when high amounts of alloreactive NK cells were infused. The aim of this study was comparing two approaches to define the size of alloreactive NK cells in haploidentical donors for AML patients recruited in two clinical trials with the acronym "NK-AML" (NCT03955848), and "MRD-NK". The standard methodology was based on the frequency of NK cell clones capable of lysing the related patient-derived cells. The alternative approach consisted of the phenotypic identification of freshly derived NK cells expressing, as inhibitory receptors, only the inhibitory KIR(s) specific for the mismatched KIR-Ligand(s) (HLA-C1, HLA-C2, HLA-Bw4). However, in KIR2DS2

Identifiants

pubmed: 36865545
doi: 10.3389/fimmu.2023.1111419
pmc: PMC9971917
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1111419

Informations de copyright

Copyright © 2023 Meazza, Ruggeri, Guolo, Minetto, Canevali, Loiacono, Ciardelli, Bo, Luchetti, Serio, Zannoni, Retière, Colomar-Carando, Parisi, Curti, Lemoli and Pende.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationship that could be construed as potential conflict of interest.

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Auteurs

Raffaella Meazza (R)

Unità Operativa UO Immunologia, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Loredana Ruggeri (L)

Divisione di Ematologia e Immunologia Clinica, Dipartimento di Medicina, Ospedale Santa Maria della Misericordia, Università di Perugia, Perugia, Italy.

Fabio Guolo (F)

Clinica di Ematologia, Dipartimento di Medicina Interna (DiMI), Università degli studi di Genova, Genova, Italy.
Dipartimento di Ematologia e Oncologia, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Paola Minetto (P)

Dipartimento di Ematologia e Oncologia, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Paolo Canevali (P)

Unità Operativa UO Immunologia, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Fabrizio Loiacono (F)

Unità Operativa UO Immunologia, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Sara Ciardelli (S)

Divisione di Ematologia e Immunologia Clinica, Dipartimento di Medicina, Ospedale Santa Maria della Misericordia, Università di Perugia, Perugia, Italy.

Alessandra Bo (A)

Laboratorio Centro Cellule Staminali e Terapie Cellulari, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Silvia Luchetti (S)

Laboratorio Centro Cellule Staminali e Terapie Cellulari, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Alberto Serio (A)

Laboratorio Centro Cellule Staminali e Terapie Cellulari, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Letizia Zannoni (L)

Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale, Università di Bologna, Bologna, Italy.

Christelle Retière (C)

Université de Nantes, Etablissement Français du Sang (EFS), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancé rologie et Immunologie Intégrée Nantes Angers (CRCI2NA), Nantes, France.

Natalia Colomar-Carando (N)

Unità Operativa UO Immunologia, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Sarah Parisi (S)

IRCCS Azienda Ospedaliero-Universitaria di Bologna, Istituto di Ematologia "Seràgnoli", Bologna, Italy.

Antonio Curti (A)

IRCCS Azienda Ospedaliero-Universitaria di Bologna, Istituto di Ematologia "Seràgnoli", Bologna, Italy.

Roberto M Lemoli (RM)

Clinica di Ematologia, Dipartimento di Medicina Interna (DiMI), Università degli studi di Genova, Genova, Italy.
Dipartimento di Ematologia e Oncologia, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Daniela Pende (D)

Unità Operativa UO Immunologia, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

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