A CUT&RUN protocol to determine patterns of epigenetic marks in imaginal discs of Drosophila.

Animals Drosophila / metabolism Drosophila melanogaster / genetics Imaginal Discs / metabolism Drosophila Proteins / genetics Epigenesis, Genetic / genetics
Bioinformatics Developmental biology Genetics Genomics Model Organisms Molecular Biology Protein Biochemistry Protein expression and purification Proteomics Sequence analysis

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
17 03 2023
Historique:
received: 14 03 2022
revised: 20 06 2022
accepted: 02 11 2022
medline: 23 10 2023
pubmed: 4 3 2023
entrez: 3 3 2023
Statut: ppublish

Résumé

Cleavage Under Targets & Release Using Nucleases (CUT&RUN) sequencing is a technique used to study gene regulation. The protocol presented here has been used successfully to identify the pattern of histone modifications within the genome of the eye-antennal disc of the fruit fly, Drosophila melanogaster. In its present form, it can be used to analyze genomic features of other imaginal discs. It can be modified for use with other tissues and applications including identifying the pattern of transcription factor occupancy.

Identifiants

DOI: 10.1016/j.xpro.2022.101878 PMID: 36867537 PMC: PMC10009584
pubmed: 36867537
pii: S2666-1667(22)00758-4
doi: 10.1016/j.xpro.2022.101878
pmc: PMC10009584
pii:
doi:

Substances chimiques

Drosophila Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

101878

Subventions

Organisme : NEI NIH HHS
ID : R01 EY030847
Pays : United States

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Brandon P Weasner (BP)

Department of Biology, Indiana University, Bloomington, IN 47405, USA.

Haley E Brown (HE)

Department of Biology, Indiana University, Bloomington, IN 47405, USA.

Robert Policastro (R)

eGenesis, Cambridge, MA 02139, USA.

Bonnie M Weasner (BM)

Department of Biology, Indiana University, Bloomington, IN 47405, USA.

Justin P Kumar (JP)

Department of Biology, Indiana University, Bloomington, IN 47405, USA. Electronic address: jkumar@indiana.edu.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › A CUT&RUN protocol to determine patterns of...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › A CUT&RUN protocol to determine patterns of...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Drosophila melanogaster › A CUT&RUN protocol to determine patterns of...
questionsmedicales.fr › Structures anatomiques de l'animal › Disques imaginaux › A CUT&RUN protocol to determine patterns of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Protéines d'insecte › Protéines de Drosophila › A CUT&RUN protocol to determine patterns of...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › A CUT&RUN protocol to determine patterns of...