Targeting osteocytes vs osteoblasts.
Cre/loxP
Differentiation
Osteoblasts
Osteocytes
Transgenic
Journal
Bone
ISSN: 1873-2763
Titre abrégé: Bone
Pays: United States
ID NLM: 8504048
Informations de publication
Date de publication:
05 2023
05 2023
Historique:
received:
17
10
2022
revised:
25
02
2023
accepted:
27
02
2023
pmc-release:
01
05
2024
pubmed:
4
3
2023
medline:
21
3
2023
entrez:
3
3
2023
Statut:
ppublish
Résumé
Although osteoblasts and osteocytes are descended from the same lineage, they each have unique and essential roles in bone. Targeting gene deletion to osteoblasts and osteocytes using the Cre/loxP system has greatly increased our current understanding of how these cells function. Additionally, the use of the Cre/loxP system in conjunction with cell-specific reporters has enabled lineage tracing of these bone cells both in vivo and ex vivo. However, concerns have been raised regarding the specificity of the promoters used and the resulting off-target effects on cells within and outside of the bone. In this review, we have summarized the main mouse models that have been used to determine the functions of specific genes in osteoblasts and osteocytes. We discuss the expression patterns and specificity of the different promoter fragments during osteoblast to osteocyte differentiation in vivo. We also highlight how their expression in non-skeletal tissues may complicate the interpretation of study results. A thorough understanding of when and where these promoters are activated will enable improved study design and greater confidence in data interpretation.
Identifiants
pubmed: 36868508
pii: S8756-3282(23)00057-1
doi: 10.1016/j.bone.2023.116724
pmc: PMC10062476
mid: NIHMS1881263
pii:
doi:
Types de publication
Review
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
116724Subventions
Organisme : NIA NIH HHS
ID : R01 AG076569
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : CommentIn
Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors have no financial competing interests to declare.
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