Wound Healing Potential of Couroupita guianensis Aubl. Fruit Pulp Investigated on Excision Wound Model.
Couroupita guianensis
Epithelization period
TGF-β
VEGF
Wound contraction
Wound healing
Journal
Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
accepted:
17
02
2023
medline:
15
11
2023
pubmed:
5
3
2023
entrez:
4
3
2023
Statut:
ppublish
Résumé
Wound care management aims at stimulating and improving healing process without scar formation. Although various plants have been reported to possess wound healing properties in tribal and folklore medicines, there is a lack of scientific data to validate the claim. In this aspect, it becomes inevitable to prove the efficacy of naturally derived products at pharmacological levels. Couroupita guianensis as a whole plant has been reported to exhibit wound healing activity. The leaves and fruit of this plant have been utilized in folkloric medicine to cure skin diseases and infections for many years. However, to the best of our knowledge, no scientific studies have been conducted to verify the wound healing properties of C. guianensis fruit pulp. Therefore, the present study seeks to investigate the wound healing potential of C. guianensis fruit pulp using an excision wound model in Wistar albino male rats. This study indicated that the ointment prepared from crude ethanolic extract of C. guianensis fruit pulp facilitated wound contraction that were evidenced by a greater reduction in the wound area and epithelialization period and increased hydroxyproline content. The experimental groups treated with low and mid dose of C. guianensis ethanol extract (CGEE) ointments had shown a wound closure of 80.27% and 89.11% respectively within 15 days, which is comparable to the standard betadine ointment which showed 91.44% healing in the treated groups. Further, the extract influenced the expression of genes VEGF and TGF-β on post wounding days that clearly explained the strong correlation between these genes and wound healing in the experimental rats. The animals treated with 10% CGEE ointment showed a significant upregulation of both VEGF and TGF-β as compared with other test and standard groups. These findings provide credence to the conventional application of this plant in the healing of wounds and other dermatological conditions, and may represent a therapeutic strategy for the treatment of wounds.
Identifiants
pubmed: 36870025
doi: 10.1007/s12010-023-04400-5
pii: 10.1007/s12010-023-04400-5
doi:
Substances chimiques
Plant Extracts
0
Ointments
0
Vascular Endothelial Growth Factor A
0
Transforming Growth Factor beta
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6516-6536Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Strodtbeck, F. (2001). Physiology of wound healing. Newborn and Infant Nursing Reviews, 1(1), 43–52.
doi: 10.1053/nbin.2001.23176
Remoue, N., Bonod, C., Fromy, B., & Sigaudo-Roussel, D. (2020). Animal models in chronic wound healing research. Innovations and Emerging Technologies in Wound Care, 197–224. https://doi.org/10.1016/b978-0-12-815028-3.00012
Velnar, T., Bailey, T., & Smrkolj. (2009). The wound healing process: An overview of the cellular and molecular mechanisms. Journal of International Medical Research, 37, 1528. https://doi.org/10.1177/147323000903700531
doi: 10.1177/147323000903700531
pubmed: 19930861
Karthick, K. G., Miraftab, M., & Ashton, J. (2010), in Medical and Healthcare Textiles: Development of a decision support system for determination of suitable dressings for wounds (Anand, S. C., Kennedy, J. F., Miraftab, M. & Rajendran, S., eds.), Woodhead Publishing, pp. 215–225. https://doi.org/10.1533/97808570 90348.215
Turner., & Badylak, S. F. (2011), Advanced Wound Repair Therapies: Engineered tissues for wound repair (David, F. ed.), Woodhead Publishing, pp. 463–494. https://doi.org/10.1533/9780857093301.4.463
Pereira, R. F., & Bartolo, P. J. (2013). Traditional therapies for skin wound healing. Advances in wound care, 5(5). https://doi.org/10.1089/wound.2013.0506
Sheba, L. A., & Anuradha, V. (2020). An updated review on Couroupita guianensis Aubl. Journal of Herbmed Pharmacology, 9, 1–11. https://doi.org/10.15171/jhp.2020.01
doi: 10.15171/jhp.2020.01
Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D. G., & Lightfoot, D. A. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6(4), 42. https://doi.org/10.3390/plants604004
doi: 10.3390/plants604004
pubmed: 28937585
pmcid: 5750618
Ingle, K. P., Deshmukh, A. G., Padole, D. A., Dudhare, M. S., Moharil, M. P., & Khelurkar, V. C. (2017). Phytochemicals: Extraction methods, identification, and detection of bioactive compounds from plant extracts. Journal Pharmacogn Phytochem., 6, 32–36.
Demirbolat, G. M., & Demirel, A. (2021). The role of ointment base on stability of dexketoprofen trometamol in ointments. Journal Research Pharmaceutical, 25(5), 681–688.
Hadi, I. A., Ugrine, H. E., Farouk, A. M., & Shayoub, M. (1989). Formulation of polyethylene glycol ointment bases suitable for tropical and subtropical climates I. Acta Pharmaceutical Hungarica, 59(3), 137–142.
Morton, J. J., & Malone, M. H. (1972). Evaluation of vulnerable activity by open wound procedure in rats. Archives Internationales de Pharmacodynamie et de Therapie, 196(1), 117–126.
pubmed: 5059357
Kokane, D. D., More, R. Y., Kale, M. B., Nehete, M. N., Mehendale, P. C., & Gadgoli, C. H. (2009). Evaluation of wound healing activity of root of Mimosa pudica. Journal of Ethnopharmacology, 124, 311–315.
doi: 10.1016/j.jep.2009.04.038
pubmed: 19397984
Pawar, R. S., Chaurasiya, P. K., Rajak, H., Singour, P. K., Toppo, F. A., & Jain, A. (2013). Wound healing activity of Sida cordifolia Linn in rats. Indian Journal of Pharmacology, 45, 474.
doi: 10.4103/0253-7613.117759
pubmed: 24130382
pmcid: 3793518
Kafel, A., Babczyńska, A., Zawisza-Raszka, A., Tarnawska, M., Płachetka-Bożek, A., & Augustyniak, M. (2021). Energy reserves, oxidative stress and development traits of Spodoptera exigua Hubner individuals from cadmium strain. Environmental Pollution, 268, 115366. https://doi.org/10.1016/j.envpol.2020.115366
doi: 10.1016/j.envpol.2020.115366
pubmed: 33035914
Aebi, H. (1984). Catalase. Methods in Enzymology, 105, 121–126.
doi: 10.1016/S0076-6879(84)05016-3
pubmed: 6727660
Murthy, S., Gautam, M. K., Goel, S., Purohit, V., Sharma, H., & Goel, R. K. (2013). Evaluation of in vivo wound healing activity of Bacopa monniera on different wound model in rats. BioMed Research International, ,1 – 9.
Reddy, G. K., & Enwemeka, C. S. (1996). A simplified method for the analysis of hydroxyproline in biological tissues. Clinical Biochemistry, 29(3), 225–229. https://doi.org/10.1016/0009-9120(96)00003-6
doi: 10.1016/0009-9120(96)00003-6
pubmed: 8740508
Ray, S., Roy, K., & Sengupta, C. (2007). In vitro evaluation of antiperoxidative potential of water extract of Spirulina platensis (blue green algae) on cyclophosphamide-induced lipid peroxidation. Indian Journal of Pharmaceutical Sciences, 69(2), 190–196.
doi: 10.4103/0250-474X.33141
Roghaye, S., Mohammad, S. I., Hamid, G., & Mahnaz, K. (2019). Expression of VEGF and TGF-β genes in skin wound healing process induced using phenytoin in male rats. Jundishapur Journal of Health Sciences, 11(1), e86041.
Boakye, Y. D., Agyare, C., Ayande, G. P., Titiloye, N., Asiamah, E. A., & Danquah, K. O. (2018). Assessment of wound-healing properties of medicinal plants: The case of Phyllanthus muellerianus. Frontiers in Pharmacology, 9. https://doi.org/10.3389/fphar.2018.00945
Umachigi, S. P., Jayaveera, K., Ashok, K. C., & Kumar, G. S. (2007). Antimicrobial, wound healing and antioxidant potential of Couroupita guianensis in rats. Pharmacologyonline, 3, 269–281.
Shrivastav, A., Mishra, A. K., Ali, S. S., Ahmad, A., Abuzinadah, M. F., & Khan, N. A. (2018). In vivo models for assesment of wound healing potential: A systematic review. Wound Medicine, 20, 43–53. https://doi.org/10.1016/j.wndm.2018.01.003
doi: 10.1016/j.wndm.2018.01.003
Nauta, A. C., Gurtner, G. C., Longaker., & M. T. (2013), in Wound Regeneration and Repair Methods and Protocols: Adult stem cells in small animal wound healing models (Gourdie R. G., & Myers T. A. eds.), Humana Press, New York, NY, pp. 81–98.
Seaton, M., Hocking, A., & Gibran, N. S. (2015). Porcine models of cutaneous wound healing. Institute for Laboratory Animal Research Journal, 56(1), 127e38.
Masson-Meyers, D. S., Andrade, T., Caetano, G. F., Guimaraes, F. R., Leite, M. N., Leite, S. N., & Frade, M. (2020). Experimental models and methods for cutaneous wound healing assessment. International Journal of Experimental Pathology, 101(1–2), 21–37. https://doi.org/10.1111/iep.12346
doi: 10.1111/iep.12346
pubmed: 32227524
pmcid: 7306904
Getie, M., Gebre-Mariam T., Rietz, R., Hohne, C., Huschka, C., Schmidtke, M., Abate, A., & Neubert R. H. H. (2003). Evaluation of the anti-microbial and anti-inflammatory activities of the medicinal plants Dodonaea viscosa, Rumex nervosus and Rumex abyssinicus. Fitoterapia,
Agarwal, P. K., Singh, A., Gaurav, K., Goel, S., Khanna, H. D., & Goel, R. K. (2009). Evaluation of wound healing activity of extracts of plantain banana (Musa sapientum var. paradisiaca) in rats. Indian Journal of Experimental Biology, 47, 32–40.
pubmed: 19317349
Nayak, B. S., Raju, S. S., Eversley, M., & Ramsubhag, A. (2009). Evaluation of wound healing activity of Lantana camara L: A preclinical study. Phytotherapy Research, 23, 241–245.
doi: 10.1002/ptr.2599
pubmed: 18844241
Dwivedi, D., Dwivedi, M., Malviya, S., & Singh, V. (2016). Evaluation of wound healing, anti-microbial and antioxidant potential of Pongamia pinnata in Wistar rats. Journal of Traditional and Complementary Medicine, 7(1), 79–85. https://doi.org/10.1016/j.jtcme.2015.12.002
doi: 10.1016/j.jtcme.2015.12.002
pubmed: 28053891
pmcid: 5198820
Suntar, I. P., Akkol, E. K., & Yilmazer, D. (2010). Investigations on the in-vivo wound healing potential of Hypericum perforatum L. Journal of Ethnopharmacology, 127, 468–477.
doi: 10.1016/j.jep.2009.10.011
pubmed: 19833187
Honnegowda, T. M., Kumar, P., Udupa, P., Rao, P., Bhandary, S., Mahato, K. K., Sharan, A., & Mayya, S. S. (2014). Effect of limited access dressing on hydroxyproline and enzymatic antioxidant status in nonhealing chronic ulcers. Indian Journal of Plastic Surgery, 47(2), 216–220. https://doi.org/10.4103/0970-0358.138952
doi: 10.4103/0970-0358.138952
pubmed: 25190917
pmcid: 4147456
Halliwell, B., Grootveld, M., & Gutteridge, J. M. (1988). Methods for the measurement of hydroxyl radicals in biomedical systems: Deoxyribose degradation and aromatic hydroxylation. Methods of Biochemical Analysis, 33, 59–90.
doi: 10.1002/9780470110546.ch2
pubmed: 2833681
Li, J., Chen, J., & Kirsner, R. (2007). Pathophysiology of acute wound healing. Clinics in Dermatology, 25, 9.
doi: 10.1016/j.clindermatol.2006.09.007
pubmed: 17276196
Brown, N. J., Smyth, E. A., Cross, S. S., & Reed, M. W. (2002). Angiogenesis induction and regression in human surgical wounds. Wound Repair and Regeneration, 10, 245.
doi: 10.1046/j.1524-475X.2002.10408.x
pubmed: 12191007
Johnson, K. E., & Wilgus, T. A. (2014). Vascular endothelial growth factor and angiogenesis in the regulation of cutaneous wound repair. Advances in Wound Care (New Rochelle), 3(10), 647–661. https://doi.org/10.1089/wound.2013.0517
doi: 10.1089/wound.2013.0517
pmcid: 4183920
Faler, B. J., Macsata, R. A., Plummer, D., Mishra, L., & Sidawy, A. N. (2006). Transforming growth factor-beta and wound healing. Perspectives in Vascular Surgery and Endovascular Therapy, 18(1), 55–62. https://doi.org/10.1177/153100350601800123
doi: 10.1177/153100350601800123
pubmed: 16628336
Kumar, I., Staton, C. A., Cross, S. S., Reed, M. W., & Brown, N. J. (2009). Angiogenesis, vascular endothelial growth factor and its receptors in human surgical wounds. British Journal of Surgery, 96, 1484–1491. https://doi.org/10.1002/bjs.6778
doi: 10.1002/bjs.6778
pubmed: 19918856