Design, synthesis, and biological testing of biphenylmethyloxazole inhibitors targeting HIV-1 reverse transcriptase.


Journal

Bioorganic & medicinal chemistry letters
ISSN: 1464-3405
Titre abrégé: Bioorg Med Chem Lett
Pays: England
ID NLM: 9107377

Informations de publication

Date de publication:
15 03 2023
Historique:
received: 17 11 2022
revised: 20 02 2023
accepted: 27 02 2023
pmc-release: 15 03 2024
pubmed: 6 3 2023
medline: 21 3 2023
entrez: 5 3 2023
Statut: ppublish

Résumé

We report non-nucleoside inhibitors of HIV-1 reverse transcriptase (NNRTIs) using a biphenylmethyloxazole pharmacophore. A crystal structure of benzyloxazole 1 was obtained and suggested the potential viability of biphenyl analogues. In particular, 6a, 6b, and 7 turned out to be potent NNRTIs with low-nanomolar activity in enzyme inhibition and infected T-cell assays, and with low cytotoxicity. Though modeling further suggested that analogues with fluorosulfate and epoxide warheads might provide covalent modification of Tyr188, synthesis and testing did not find evidence for this outcome.

Identifiants

pubmed: 36871704
pii: S0960-894X(23)00094-X
doi: 10.1016/j.bmcl.2023.129216
pmc: PMC10278203
mid: NIHMS1882221
pii:
doi:

Substances chimiques

reverse transcriptase, Human immunodeficiency virus 1 EC 2.7.7.-
Reverse Transcriptase Inhibitors 0
HIV Reverse Transcriptase EC 2.7.7.49
Anti-HIV Agents 0

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

129216

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI155072
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM049551
Pays : United States

Informations de copyright

Copyright © 2023 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Auteurs

Zachary J Carter (ZJ)

Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA.

Klarissa Hollander (K)

Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520-8066, USA; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520-8066, USA.

Krasimir A Spasov (KA)

Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520-8066, USA.

Karen S Anderson (KS)

Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520-8066, USA; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520-8066, USA. Electronic address: karen.anderson@yale.edu.

William L Jorgensen (WL)

Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA. Electronic address: william.jorgensen@yale.edu.

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Classifications MeSH