Biglycan regulates bone development and regeneration.

biglycan bone cartilage extracellular matrix fracture periosteum

Journal

Frontiers in physiology
ISSN: 1664-042X
Titre abrégé: Front Physiol
Pays: Switzerland
ID NLM: 101549006

Informations de publication

Date de publication:
2023
Historique:
received: 08 12 2022
accepted: 31 01 2023
entrez: 6 3 2023
pubmed: 7 3 2023
medline: 7 3 2023
Statut: epublish

Résumé

Endochondral bone development and regeneration relies on activation and proliferation of periosteum derived-cells (PDCs). Biglycan (Bgn), a small proteoglycan found in extracellular matrix, is known to be expressed in bone and cartilage, however little is known about its influence during bone development. Here we link biglycan with osteoblast maturation starting during embryonic development that later affects bone integrity and strength. Biglycan gene deletion reduced the inflammatory response after fracture, leading to impaired periosteal expansion and callus formation. Using a novel 3D scaffold with PDCs, we found that biglycan could be important for the cartilage phase preceding bone formation. The absence of biglycan led to accelerated bone development with high levels of osteopontin, which appeared to be detrimental to the structural integrity of the bone. Collectively, our study identifies biglycan as an influencing factor in PDCs activation during bone development and bone regeneration after fracture.

Identifiants

pubmed: 36875017
doi: 10.3389/fphys.2023.1119368
pii: 1119368
pmc: PMC9979216
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1119368

Informations de copyright

Copyright © 2023 Shainer, Kram, Kilts, Li, Doyle, Shainer, Martin, Simon, Zeng-Brouwers, Schaefer, Young and Genomics and Computational.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Reut Shainer (R)

Molecular Biology of Bones and Teeth Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, United States.

Vardit Kram (V)

Molecular Biology of Bones and Teeth Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, United States.

Tina M Kilts (TM)

Molecular Biology of Bones and Teeth Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, United States.

Li Li (L)

Molecular Biology of Bones and Teeth Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, United States.

Andrew D Doyle (AD)

NIDCR Imaging Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, United States.

Inbal Shainer (I)

Department Genes-Circuits-Behavior, Max Planck Institute for Biological Intelligence, Martinsried, Germany.

Daniel Martin (D)

NIDCD/NIDCR Genomics and Computational Biology Core, National Institutes of Health, Bethesda, MD, United States.

Carl G Simon (CG)

Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, MD, United States.

Jinyang Zeng-Brouwers (J)

Pharmazentrum Frankfurt, Institut für Allgemeine Pharmakologie und Toxikologie, Klinikum der Goethe-Universität Frankfurt am Main, Frankfurt, Germany.

Liliana Schaefer (L)

Pharmazentrum Frankfurt, Institut für Allgemeine Pharmakologie und Toxikologie, Klinikum der Goethe-Universität Frankfurt am Main, Frankfurt, Germany.

Marian F Young (MF)

Molecular Biology of Bones and Teeth Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, United States.

Classifications MeSH