A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue.
Alzheimer's disease
HPLC-MS/MS
Hippocampus
Neurotransmitter
Parkinson's disease
Rat
Striatum
Journal
MethodsX
ISSN: 2215-0161
Titre abrégé: MethodsX
Pays: Netherlands
ID NLM: 101639829
Informations de publication
Date de publication:
2023
2023
Historique:
received:
10
11
2022
accepted:
13
02
2023
entrez:
6
3
2023
pubmed:
7
3
2023
medline:
7
3
2023
Statut:
epublish
Résumé
The detection of neurotransmitters has extensively been applied to the study of the pathogenesis, diagnosis, and therapeutic effect of drugs on many neuropsychiatric diseases. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been employed to determine neurotransmitters levels due to its distinct advantages. However, neurotransmitter detection still presents some challenges. A rapid and sensitive HPLC-MS/MS protocol has been established in our lab, which can simultaneously detect 5 neurotransmitters with an easy pretreatment procedure. The protocol provides demanded reference value for the lab using an Agilent HPLC-MS/MS system with a triple quadrupole analyzer.
Identifiants
pubmed: 36875344
doi: 10.1016/j.mex.2023.102083
pii: S2215-0161(23)00086-9
pmc: PMC9978030
doi:
Types de publication
Journal Article
Langues
eng
Pagination
102083Informations de copyright
© 2023 The Authors. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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