Extraction, physicochemical properties, and antioxidant activity of natural melanin from
Auricularia heimuer
HPLC
antioxidant activities
natural melanin
physicochemical properties
Journal
Frontiers in nutrition
ISSN: 2296-861X
Titre abrégé: Front Nutr
Pays: Switzerland
ID NLM: 101642264
Informations de publication
Date de publication:
2023
2023
Historique:
received:
25
12
2022
accepted:
30
01
2023
entrez:
6
3
2023
pubmed:
7
3
2023
medline:
7
3
2023
Statut:
epublish
Résumé
Natural melanin from In this study, single-factor experiments, Box-Behnken design (BBD), and response surface methodology (RSM) were employed to investigate the effects of alkali-soluble pH, acid precipitation pH, and microwave time on the extraction yield of The results showed that alkali-soluble pH, acid precipitation pH, and microwave time significantly affected the AHM yield, with the following optimized microwave-assisted extraction conditions: alkali-soluble pH of 12.3, acid precipitation pH of 3.1, and microwave time of 53 min, resulting in an AHM extraction yield of 0.4042%. AHM exhibited a strong absorption at 210 nm, similar to melanin from other sources. FT-IR spectroscopy also revealed that AHM exhibited the three characteristic absorption peaks of natural melanin. The HPLC chromatogram profile of AHM showed a single symmetrical elution peak with a 2.435 min retention time. AHM was highly soluble in alkali solution, insoluble in distilled water and organic solvents, and demonstrated strong DPPH, OH, and ABTS free radical scavenging activities. This study provides technical support to optimize AHM extraction for use in the medical and food industries.
Identifiants
pubmed: 36875843
doi: 10.3389/fnut.2023.1131542
pmc: PMC9981798
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1131542Informations de copyright
Copyright © 2023 Ma, Zhang, Dai, Yao, Zhou, Ma, Liu, Tian, Zhu, Zhang, Kong and Bao.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Carbohydr Polym. 2016 Jun 5;143:100-7
pubmed: 27083348
Dokl Biol Sci. 2021 Sep;500(1):159-169
pubmed: 34731382
Nat Prod Res. 2021 Oct;35(20):3417-3425
pubmed: 31829039
PLoS One. 2015 Nov 10;10(11):e0142439
pubmed: 26555720
Genomics. 2019 Jan;111(1):50-58
pubmed: 29288711
Food Chem. 2016 Jan 1;190:801-807
pubmed: 26213041
Front Nutr. 2022 Apr 18;9:862773
pubmed: 35520284
Carbohydr Polym. 2020 Nov 1;247:116750
pubmed: 32829866
Food Funct. 2019 Feb 20;10(2):1017-1027
pubmed: 30706914
Carbohydr Polym. 2012 Jun 20;89(2):694-700
pubmed: 24750775
World J Microbiol Biotechnol. 2020 Oct 12;36(11):170
pubmed: 33043393
FEMS Microbiol Lett. 2018 Oct 1;365(19):
pubmed: 29912354
Medchemcomm. 2018 May 16;9(6):1059-1068
pubmed: 30108995
Front Microbiol. 2022 Nov 10;13:1048249
pubmed: 36439811
Int J Med Mushrooms. 2021;23(6):89-100
pubmed: 34369737
Food Funct. 2016 Aug 10;7(8):3617-27
pubmed: 27485489
Food Sci Nutr. 2019 Oct 21;7(11):3774-3783
pubmed: 31763027
Appl Biochem Biotechnol. 2015 May;176(1):253-66
pubmed: 25800528
Food Chem. 2019 Mar 30;277:533-542
pubmed: 30502181
J Basic Microbiol. 2022 Jul;62(7):843-856
pubmed: 35419841
Mitochondrial DNA B Resour. 2019 Nov 13;4(2):4029-4030
pubmed: 33366302
Food Chem. 2016 Apr 1;196:486-92
pubmed: 26593518
World J Microbiol Biotechnol. 2022 Sep 23;38(12):228
pubmed: 36149606
Appl Biochem Biotechnol. 2014 Sep;174(2):762-71
pubmed: 25091327
Int J Biol Macromol. 2020 Jun 1;152:1157-1163
pubmed: 31751731
Foods. 2021 Oct 14;10(10):
pubmed: 34681485
Foods. 2021 Nov 23;10(12):
pubmed: 34945444
Food Chem. 2022 Mar 15;372:131171
pubmed: 34601416
Molecules. 2019 Apr 05;24(7):
pubmed: 30959759
Ukr Biokhim Zh (1999). 1999 Jul-Aug;71(4):5-14
pubmed: 10791050
Front Microbiol. 2021 Jan 15;11:610173
pubmed: 33519763
Sci Rep. 2019 Feb 11;9(1):1724
pubmed: 30741980
Int J Biol Macromol. 2020 May 1;150:102-113
pubmed: 32044370
J Appl Biomater Funct Mater. 2022 Jan-Dec;20:22808000221124418
pubmed: 36254348
Front Nutr. 2022 Feb 28;9:858673
pubmed: 35295915
Mater Sci Eng C Mater Biol Appl. 2019 Jan 1;94:17-25
pubmed: 30423699
Sci Rep. 2022 Feb 8;12(1):2110
pubmed: 35136095
Appl Biochem Biotechnol. 2019 Jun;188(2):555-567
pubmed: 30547280
Int J Med Mushrooms. 2018;20(11):1087-1095
pubmed: 30806232
Mycology. 2018 Jul 09;9(4):296-306
pubmed: 30533254
Bioorg Med Chem Lett. 2017 Apr 15;27(8):1831-1834
pubmed: 28284807
Int J Med Mushrooms. 2022;24(1):67-82
pubmed: 35442596
J Bacteriol. 1993 Nov;175(21):7102-4
pubmed: 8226653
Biotechnol Adv. 2021 Dec;53:107773
pubmed: 34022328