Regulation of pSYSA defense plasmid copy number in

CRISPR RNase E antisense RNA cyanobacteria expression vectors plasmid replication replication protein

Journal

Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977

Informations de publication

Date de publication:
2023
Historique:
received: 30 11 2022
accepted: 23 01 2023
entrez: 6 3 2023
pubmed: 7 3 2023
medline: 7 3 2023
Statut: epublish

Résumé

Synthetic biology approaches toward the development of cyanobacterial producer strains require the availability of appropriate sets of plasmid vectors. A factor for the industrial usefulness of such strains is their robustness against pathogens, such as bacteriophages infecting cyanobacteria. Therefore, it is of great interest to understand the native plasmid replication systems and the CRISPR-Cas based defense mechanisms already present in cyanobacteria. In the model cyanobacterium

Identifiants

pubmed: 36876071
doi: 10.3389/fmicb.2023.1112307
pmc: PMC9978351
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1112307

Informations de copyright

Copyright © 2023 Kaltenbrunner, Reimann, Hoffmann, Aoyagi, Sakata, Nimura-Matsune, Watanabe, Steglich, Wilde and Hess.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Alena Kaltenbrunner (A)

Genetics and Experimental Bioinformatics Group, Faculty of Biology, University of Freiburg, Freiburg, Germany.

Viktoria Reimann (V)

Genetics and Experimental Bioinformatics Group, Faculty of Biology, University of Freiburg, Freiburg, Germany.

Ute A Hoffmann (UA)

Molecular Genetics of Prokaryotes, Institute of Biology III, University of Freiburg, Freiburg, Germany.

Tomohiro Aoyagi (T)

Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.

Minori Sakata (M)

Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.

Kaori Nimura-Matsune (K)

Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.

Satoru Watanabe (S)

Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.

Claudia Steglich (C)

Genetics and Experimental Bioinformatics Group, Faculty of Biology, University of Freiburg, Freiburg, Germany.

Annegret Wilde (A)

Molecular Genetics of Prokaryotes, Institute of Biology III, University of Freiburg, Freiburg, Germany.

Wolfgang R Hess (WR)

Genetics and Experimental Bioinformatics Group, Faculty of Biology, University of Freiburg, Freiburg, Germany.

Classifications MeSH