Novel canonical and non-canonical viral antigens extend current targets for immunotherapy of HPV-driven cervical cancer.
Cancer
Cell biology
Immunology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
17 Mar 2023
17 Mar 2023
Historique:
received:
26
06
2022
revised:
30
08
2022
accepted:
26
01
2023
entrez:
6
3
2023
pubmed:
7
3
2023
medline:
7
3
2023
Statut:
epublish
Résumé
Current immunotherapeutic approaches for human papillomavirus (HPV)-driven cervical cancer target the viral oncogenes E6 and E7. We report viral canonical and alternative reading frame (ARF)-derived sequences presented on cervical tumor cells, including antigens encoded by the conserved viral gene E1. We confirm immunogenicity of the identified viral peptides in HPV-positive women, and women with cervical intraepithelial neoplasia. We observe consistent transcription of the E1, E6, and E7 genes in 10 primary cervical tumor resections from the four most common high-risk HPV subtypes (HPV16, 18, 31, and 45), suggesting the suitability of E1 as therapeutic target. We finally confirm HLA presentation of canonical peptides derived from E6 and E7, and ARF-derived viral peptides from a reverse-strand transcript spanning the HPV E1 and E2 genes in primary human cervical tumor tissue. Our results extend currently known viral immunotherapeutic targets in cervical cancer and highlight E1 as an important cervical cancer antigen.
Identifiants
pubmed: 36876126
doi: 10.1016/j.isci.2023.106101
pii: S2589-0042(23)00178-5
pmc: PMC9978627
doi:
Types de publication
Journal Article
Langues
eng
Pagination
106101Informations de copyright
© 2023 The Author(s).
Déclaration de conflit d'intérêts
N.T. is or has been scientific advisor to Enara Bio, Grey Wolf Therapeutics, Roche Pharma, Infinitopes Ltd., and T-Cypher Bio.
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