Comprehensive characterization of the embryonic factor LEUTX.
Molecular biology
Omics
Reproductive medicine
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
17 Mar 2023
17 Mar 2023
Historique:
received:
07
07
2022
revised:
01
12
2022
accepted:
06
02
2023
entrez:
6
3
2023
pubmed:
7
3
2023
medline:
7
3
2023
Statut:
epublish
Résumé
The paired-like homeobox transcription factor LEUTX is expressed in human preimplantation embryos between the 4- and 8-cell stages, and then silenced in somatic tissues. To characterize the function of LEUTX, we performed a multiomic characterization of LEUTX using two proteomics methods and three genome-wide sequencing approaches. Our results show that LEUTX stably interacts with the EP300 and CBP histone acetyltransferases through its 9 amino acid transactivation domain (9aaTAD), as mutation of this domain abolishes the interactions. LEUTX targets genomic cis-regulatory sequences that overlap with repetitive elements, and through these elements it is suggested to regulate the expression of its downstream genes. We find LEUTX to be a transcriptional activator, upregulating several genes linked to preimplantation development as well as 8-cell-like markers, such as DPPA3 and ZNF280A. Our results support a role for LEUTX in preimplantation development as an enhancer binding protein and as a potent transcriptional activator.
Identifiants
pubmed: 36876139
doi: 10.1016/j.isci.2023.106172
pii: S2589-0042(23)00249-3
pmc: PMC9978639
doi:
Types de publication
Journal Article
Langues
eng
Pagination
106172Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
Y.M. is an inventor on a patent related to NET-CAGE technology. Other authors declare no competing interest.
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