Protein interactors of 3-O sulfated heparan sulfates in human MCI and age-matched control cerebrospinal fluid.
Journal
Scientific data
ISSN: 2052-4463
Titre abrégé: Sci Data
Pays: England
ID NLM: 101640192
Informations de publication
Date de publication:
06 03 2023
06 03 2023
Historique:
received:
06
09
2022
accepted:
07
02
2023
entrez:
6
3
2023
pubmed:
7
3
2023
medline:
9
3
2023
Statut:
epublish
Résumé
Heparan sulfates (HS) proteoglycans are commonly found on the cell surface and mediate many processes. Binding of HS ligands is determined by the sulfation code on the HS chain that can be N-/2-O/6-O- or 3-O-sulfated, generating heterogenous sulfation patterns. 3-O sulfated HS (3S-HS) play a role in several (patho)physiological processes such as blood coagulation, viral pathogenesis and binding and internalization of tau in Alzheimer's disease. However, few 3S-HS-specific interactors are known. Thus, our insight into the role of 3S-HS in health and disease is limited, especially in the central nervous system. Using human CSF, we determined the interactome of synthetic HS with defined sulfation patterns. Our affinity-enrichment mass spectrometry studies expand the repertoire of proteins that may interact with (3S-)HS. Validating our approach, ATIII, a known 3S-HS interactor, was found to require GlcA-GlcNS6S3S for binding, similar to what has been reported. Our dataset holds novel, potential HS and 3S-HS protein ligands, that can be explored in future studies focusing on molecular mechanisms that depend on 3S-HS in (patho)physiological conditions.
Identifiants
pubmed: 36879013
doi: 10.1038/s41597-023-02009-1
pii: 10.1038/s41597-023-02009-1
pmc: PMC9986659
doi:
Substances chimiques
Heparitin Sulfate
9050-30-0
Ligands
0
Sulfates
0
Types de publication
Dataset
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
121Subventions
Organisme : Agentschap Innoveren en Ondernemen (Flanders Innovation & Entrepreneurship)
ID : HBC.2017.0231
Informations de copyright
© 2023. The Author(s).
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