Amnion-derived serum amyloid A1 participates in sterile inflammation of fetal membranes at parturition.
Amnion
Chemotaxis
Fetal membranes
Parturition
Serum amyloid A1
Sterile inflammation
Journal
Inflammation research : official journal of the European Histamine Research Society ... [et al.]
ISSN: 1420-908X
Titre abrégé: Inflamm Res
Pays: Switzerland
ID NLM: 9508160
Informations de publication
Date de publication:
Apr 2023
Apr 2023
Historique:
received:
04
01
2023
accepted:
19
02
2023
revised:
05
02
2023
medline:
27
4
2023
pubmed:
7
3
2023
entrez:
6
3
2023
Statut:
ppublish
Résumé
Sterile inflammation of fetal membranes is an indispensable event of normal parturition. However, triggers of sterile inflammation are not fully resolved. Serum amyloid A1 (SAA1) is an acute phase protein produced primarily by the liver. Fetal membranes can also synthesize SAA1 but its functions are not well defined. Given the role of SAA1 in the acute phase response to inflammation, we postulated that SAA1 synthesized in the fetal membranes may be a trigger of local inflammation at parturition. The changes of SAA1 abundance in parturition were studied in the amnion of human fetal membranes. The role of SAA1 in chemokine expression and leukocyte chemotaxis was examined in cultured human amnion tissue explants as well as primary human amnion fibroblasts. The effects of SAA1 on monocytes, macrophages and dendritic cells were investigated in cells derived from a human leukemia monocytic cell line (THP-1). SAA1 synthesis increased significantly in human amnion at parturition. SAA1 evoked multiple chemotaxis pathways in human amnion fibroblasts along with upregulation of a series of chemokines via both toll-like receptor 4 (TLR4) and formyl peptide receptor 2 (FPR2). Moreover, SAA1-conditioned medium of cultured amnion fibroblasts was capable of chemoattracting virtually all types of mononuclear leukocytes, particularly monocytes and dendritic cells, which reconciled with the chemotactic activity of conditioned medium of cultured amnion tissue explants collected from spontaneous labor. Furthermore, SAA1 could induce the expression of genes associated with inflammation and extracellular matrix remodeling in monocytes, macrophages and dendritic cells derived from THP-1. SAA1 is a trigger of sterile inflammation of the fetal membranes at parturition.
Identifiants
pubmed: 36879064
doi: 10.1007/s00011-023-01713-3
pii: 10.1007/s00011-023-01713-3
doi:
Substances chimiques
Culture Media, Conditioned
0
Chemokines
0
Serum Amyloid A Protein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
797-812Subventions
Organisme : National Natural Science Foundation of China
ID : 81830042
Organisme : National Key Research and Development Program of China
ID : 2022YFC2704602
Organisme : Innovative Research Team of High-level Local Universities in Shanghai
ID : SHSMU-ZLCX20210201
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
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