Ex vivo detection of lipopolysaccharide immunopositivity in Rushton bodies.

Humans Radicular Cyst / pathology Lipopolysaccharides Epithelium / pathology Calcinosis / pathology

Journal

Histology and histopathology
ISSN: 1699-5848
Titre abrégé: Histol Histopathol
Pays: Spain
ID NLM: 8609357

Informations de publication

Date de publication:
Aug 2023
Historique:
medline: 17 7 2023
pubmed: 8 3 2023
entrez: 7 3 2023
Statut: ppublish

Résumé

Our aim was to investigate how bacterial lipopolysaccharide (LPS) is immunoexpressed in periapical lesions. By surprise we detected Rushton bodies (RBs) whose origin has been debatable to be positive for LPS. Samples of radicular cysts (N=70) were stained in order to identify variations in LPS immunoexpression indicating bacterial background. For immunostaining, we used an anti-LPS antibody from Escherichia coli, and for visualization Horse Radish Peroxidase labeled polymer as the secondary antibody. RBs showed positivity for LPS in radicular cysts. After collection of radicular cyst samples (70 in total), we noted that all RBs (N=25) histologically detected in tissue samples were positive for LPS. Furthermore, calcification in the cyst capsule showed immunopositivity. We demonstrate for the first time that LPS is present in RBs, indicating that host response to bacteria might be the initial cause of the formation of these hyaline bodies in the cyst epithelium and cyst capsule calcifications.

Identifiants

DOI: 10.14670/HH-18-602 PMID: 36880590
pubmed: 36880590
pii: HH-18-602
doi: 10.14670/HH-18-602
doi:

Substances chimiques

Lipopolysaccharides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

889-892

Subventions

Organisme : Finnish Female dentist's association and HUS research
ID : Y780022044

Informations de copyright

©The Author(s) 2023. Open Access. This article is licensed under a Creative Commons CC-BY International License.

Références

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Auteurs

Sirke Virkkunen (S)

Department of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. sirke.virkkunen@helsinki.fi.

Jaana Willberg (J)

Department of Oral Pathology and Radiology, University of Turku, Turku, Finland.

Caj Haglund (C)

Department of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Department of Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Translational Cancer Medicine Research Program, Faculty of Medicine, University of Helsinki, Finland.

Malin Sund (M)

Department of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Department of Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.

Timo Sorsa (T)

Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Section of Periodontology and Dental Prevention, Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden.

Jaana Hagström (J)

Department of Oral Pathology and Radiology, University of Turku, Turku, Finland.
Department of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Translational Cancer Medicine Research Program, Faculty of Medicine, University of Helsinki, Finland.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Ex vivo detection of lipopolysaccharide...
questionsmedicales.fr › Maladies du système stomatognathique › Maladies de la bouche › Maladies parodontales › Maladies périapicales › Kyste radiculaire › Ex vivo detection of lipopolysaccharide...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Toxines bactériennes › Endotoxines › Lipopolysaccharides › Ex vivo detection of lipopolysaccharide...
questionsmedicales.fr › Tissus › Épithélium › Ex vivo detection of lipopolysaccharide...
questionsmedicales.fr › Maladies métaboliques et nutritionnelles › Maladies métaboliques › Troubles du métabolisme du calcium › Calcinose › Ex vivo detection of lipopolysaccharide...