Localization and expression of SLX4 in the testis of sterile male cattle-yak.
SLX4
hybrid sterility
meiosis
spermatocyte
spermatogenesis
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
revised:
22
02
2023
received:
27
11
2022
accepted:
03
03
2023
medline:
4
5
2023
pubmed:
8
3
2023
entrez:
7
3
2023
Statut:
ppublish
Résumé
Cattle-yak, the hybrid offspring of yak (Bos grunniens) and cattle (Bos taurus), serves as a unique model to dissect the molecular mechanisms underlying reproductive isolation. While female cattle-yaks are fertile, the males are completely sterile due to spermatogenic arrest at the meiosis stage and massive germ cell apoptosis. Interestingly, meiotic defects are partially rescued in the testes of backcrossed offspring. The genetic basis of meiotic defects in male cattle-yak remains unclear. Structure-specific endonuclease subunit (SLX4) participates in meiotic double-strand break (DSB) formation in mice, and its deletion results in defects in spermatogenesis. In the present study, we examined the expression patterns of SLX4 in the testes of yak, cattle-yak, and backcrossed offspring to investigate its potential roles in hybrid sterility. The results showed that the relative abundances of SLX4 mRNA and protein were significantly reduced in the testis of cattle-yak. The results of immunohistochemistry revealed that SLX4 was predominately expressed in spermatogonia and spermatocytes. Chromosome spreading experiments showed that SLX4 was significantly decreased in the pachytene spermatocytes of cattle-yak compared with yak and backcrossed offspring. These findings suggest that SLX4 expression was dysregulated in the testis of cattle-yak, potentially resulting in the failure of crossover formation and collapses of meiosis in hybrid males.
Substances chimiques
Recombinases
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
679-687Subventions
Organisme : National Key Research and Development Project
ID : 2021YFD1600201
Organisme : Strategic Priority Research Program of CAS
ID : XDA2004010303
Organisme : National Natural Science Foundation of China
ID : 31972574
Organisme : Natural Science Foundation of Qinghai
ID : 2019-ZJ-902
Organisme : Youth Innovation Promotion Association CAS
ID : 2021432
Informations de copyright
© 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.
Références
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