A Reproducible Spinal Cord Crush Injury in the Regeneration-Permissive Axolotl.
Axolotl
Crush injury
Spinal cord
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
7
3
2023
pubmed:
8
3
2023
medline:
10
3
2023
Statut:
ppublish
Résumé
Following injury, axolotls are able to functionally regenerate their spinal cord, regaining both motor and sensory control. In contrast, humans respond to severe spinal cord injury by forming a glial scar, which prevents further damage but also inhibits any regenerative growth, resulting in loss of function caudal to the injury site. The axolotl has become a popular system to elucidate the underlying cellular and molecular events that contribute to successful CNS regeneration. However, the experimental injuries (tail amputation and transection) that are utilized in axolotls do not mimic the blunt trauma that is often sustained in humans. Here, we report a more clinically relevant model for spinal cord injuries in the axolotl using a weight-drop technique. This reproducible model allows precise control over the severity of the injury by regulating the drop height, weight, compression, and position of the injury.
Identifiants
pubmed: 36881304
doi: 10.1007/978-1-0716-3012-9_13
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
237-246Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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