Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters - a rat study.
Histopathology
Osteocyte lacuna
Osteonecrosis
Temperature
Journal
Head & face medicine
ISSN: 1746-160X
Titre abrégé: Head Face Med
Pays: England
ID NLM: 101245792
Informations de publication
Date de publication:
07 Mar 2023
07 Mar 2023
Historique:
received:
16
09
2022
accepted:
25
01
2023
entrez:
7
3
2023
pubmed:
8
3
2023
medline:
10
3
2023
Statut:
epublish
Résumé
This rat study aimed to evaluate the feasibility of temperature thresholds that affect peri-implant bone cells and morphology and the potential usefulness of thermal necrosis for inducing implant removal for a subsequent in vivo pig study. On one side, rat tibiae were thermally treated before implant insertion. The contralateral side was used as the control group without tempering. Temperatures of 4 °C, 3 °C, 2 °C, 48 °C, 49 °C, and 50 °C were evaluated with a tempering time of 1 min. Energy-dispersive X-ray spectroscopy (EDX) and transmission electron microscopy (TEM) analyses were performed. The EDX analysis revealed significant increases in element weights at 50 °C (e.g., calcium, phosphate, sodium, and sulfur; p < 0.01). The results of the TEM analysis showed that at all the applied cold and warm temperatures, signs of cell damage were observed, including vacuolization, shrinkage, and detachment from the surrounding bone matrix. Some cells became necrotic, leaving the lacunae empty. Temperature of 50 °C led to irreversible cell death. The degree of damage was more significant at 50 °C and 2 °C than at 48 °C and 5 °C. Although this was a preliminary study, from the results, we identified that a temperature of 50 °C at a time interval of 60 min can lower the number of samples in a further study of thermo-explantation. Thus, the subsequent planned in vivo study in pigs, which will consider osseointegrated implants, is feasible.
Sections du résumé
BACKGROUND
BACKGROUND
This rat study aimed to evaluate the feasibility of temperature thresholds that affect peri-implant bone cells and morphology and the potential usefulness of thermal necrosis for inducing implant removal for a subsequent in vivo pig study.
METHODS
METHODS
On one side, rat tibiae were thermally treated before implant insertion. The contralateral side was used as the control group without tempering. Temperatures of 4 °C, 3 °C, 2 °C, 48 °C, 49 °C, and 50 °C were evaluated with a tempering time of 1 min. Energy-dispersive X-ray spectroscopy (EDX) and transmission electron microscopy (TEM) analyses were performed.
RESULTS
RESULTS
The EDX analysis revealed significant increases in element weights at 50 °C (e.g., calcium, phosphate, sodium, and sulfur; p < 0.01). The results of the TEM analysis showed that at all the applied cold and warm temperatures, signs of cell damage were observed, including vacuolization, shrinkage, and detachment from the surrounding bone matrix. Some cells became necrotic, leaving the lacunae empty.
CONCLUSIONS
CONCLUSIONS
Temperature of 50 °C led to irreversible cell death. The degree of damage was more significant at 50 °C and 2 °C than at 48 °C and 5 °C. Although this was a preliminary study, from the results, we identified that a temperature of 50 °C at a time interval of 60 min can lower the number of samples in a further study of thermo-explantation. Thus, the subsequent planned in vivo study in pigs, which will consider osseointegrated implants, is feasible.
Identifiants
pubmed: 36882765
doi: 10.1186/s13005-023-00349-2
pii: 10.1186/s13005-023-00349-2
pmc: PMC9990269
doi:
Substances chimiques
Dental Implants
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4Subventions
Organisme : AiF Projekt
ID : research project 20302N
Organisme : AiF Projekt
ID : research project 20302N
Organisme : AiF Projekt
ID : research project 20302N
Organisme : AiF Projekt
ID : research project 20302N
Informations de copyright
© 2023. The Author(s).
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