Transcriptomic analysis of the Non-Obstructive Azoospermia (NOA) to address gene expression regulation in human testis.

RNA-seq Spermatogenesis gene expression regulation male infertility molecular interactions non-obstructive Azoospermia regulatory network transcription factors transcription regulation transcriptomics

Journal

Systems biology in reproductive medicine
ISSN: 1939-6376
Titre abrégé: Syst Biol Reprod Med
Pays: England
ID NLM: 101464963

Informations de publication

Date de publication:
Jun 2023
Historique:
medline: 15 5 2023
pubmed: 9 3 2023
entrez: 8 3 2023
Statut: ppublish

Résumé

There is a need to understand the molecular basis of testes under Non-Obstructive Azoospermia (NOA), a state of failed spermatogenesis. There has been a lack of attention to the transcriptome at the level of alternatively spliced mRNAs (iso-mRNAs) and the mechanism of gene expression regulation. Hence, we aimed to establish a reliable iso-mRNA profile of NOA-testes, and explore molecular mechanisms - especially those related to gene expression regulation. We sequenced mRNAs from testicular samples of donors with complete spermatogenesis (control samples) and a failure of spermatogenesis (NOA samples). We identified differentially expressed genes and their iso-mRNAs

Identifiants

pubmed: 36883778
doi: 10.1080/19396368.2023.2176268
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

196-214

Auteurs

Govindkumar Balagannavar (G)

Institute of Bioinformatics and Applied Biotechnology (IBAB), Bengaluru, Karnataka, India.
Research Scholar, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India.

Kavyashree Basavaraju (K)

Institute of Bioinformatics and Applied Biotechnology (IBAB), Bengaluru, Karnataka, India.
BdataA: Biological data Analyzers' Association (virtual organization http://startbioinfo.com/BdataA/), India.

Akhilesh Kumar Bajpai (AK)

BdataA: Biological data Analyzers' Association (virtual organization http://startbioinfo.com/BdataA/), India.

Sravanthi Davuluri (S)

BdataA: Biological data Analyzers' Association (virtual organization http://startbioinfo.com/BdataA/), India.

Shruthi Kannan (S)

Institute of Bioinformatics and Applied Biotechnology (IBAB), Bengaluru, Karnataka, India.

Vasan S Srini (V)

Manipal Fertility, Manipal Hospital, Bengaluru, Karnataka, India.

Darshan S Chandrashekar (D)

Institute of Bioinformatics and Applied Biotechnology (IBAB), Bengaluru, Karnataka, India.

Neelima Chitturi (N)

BdataA: Biological data Analyzers' Association (virtual organization http://startbioinfo.com/BdataA/), India.

Kshitish K Acharya (K)

Institute of Bioinformatics and Applied Biotechnology (IBAB), Bengaluru, Karnataka, India.
BdataA: Biological data Analyzers' Association (virtual organization http://startbioinfo.com/BdataA/), India.

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Classifications MeSH