Direct observation of motor protein stepping in living cells using MINFLUX.
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
10 03 2023
10 03 2023
Historique:
entrez:
9
3
2023
pubmed:
10
3
2023
medline:
14
3
2023
Statut:
ppublish
Résumé
Dynamic measurements of molecular machines can provide invaluable insights into their mechanism, but these measurements have been challenging in living cells. Here, we developed live-cell tracking of single fluorophores with nanometer spatial and millisecond temporal resolution in two and three dimensions using the recently introduced super-resolution technique MINFLUX. Using this approach, we resolved the precise stepping motion of the motor protein kinesin-1 as it walked on microtubules in living cells. Nanoscopic tracking of motors walking on the microtubules of fixed cells also enabled us to resolve the architecture of the microtubule cytoskeleton with protofilament resolution.
Identifiants
pubmed: 36893247
doi: 10.1126/science.ade2676
pmc: PMC7614483
mid: EMS173946
doi:
Substances chimiques
Fluorescent Dyes
0
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1010-1015Subventions
Organisme : European Research Council
ID : 724489
Pays : International
Organisme : European Research Council
ID : 819219
Pays : International
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