Potential biomarker proteins for aspiration pneumonia detected by shotgun proteomics using buccal mucosa samples: a cross-sectional case-control study.
Aspiration pneumonia
Biomarker
Buccal mucosa
Proteomics
Journal
Clinical proteomics
ISSN: 1542-6416
Titre abrégé: Clin Proteomics
Pays: England
ID NLM: 101184586
Informations de publication
Date de publication:
09 Mar 2023
09 Mar 2023
Historique:
received:
15
06
2022
accepted:
21
02
2023
entrez:
9
3
2023
pubmed:
10
3
2023
medline:
10
3
2023
Statut:
epublish
Résumé
Aspiration pneumonia (AP), which is a major cause of death in the elderly, does present with typical symptoms in the early stages of onset, thus it is difficult to detect and treat at an early stage. In this study, we identified biomarkers that are useful for the detection of AP and focused on salivary proteins, which may be collected non-invasively. Because expectorating saliva is often difficult for elderly people, we collected salivary proteins from the buccal mucosa. We collected samples from the buccal mucosa of six patients with AP and six control patients (no AP) in an acute-care hospital. Following protein precipitation using trichloroacetic acid and washing with acetone, the samples were analyzed by liquid chromatography and tandem mass spectrometry (LC-MS/MS). We also determined the levels of cytokines and chemokines in non-precipitated samples from buccal mucosa. Comparative quantitative analysis of LC-MS/MS spectra revealed 55 highly (P values < 0.10) abundant proteins with high FDR confidence (q values < 0.01) and high coverage (> 50%) in the AP group compared with the control group. Among the 55 proteins, the protein abundances of four proteins (protein S100-A7A, eukaryotic translation initiation factor 1, Serpin B4, and peptidoglycan recognition protein 1) in the AP group showed a negative correlation with the time post-onset; these proteins are promising AP biomarker candidates. In addition, the abundance of C-reactive protein (CRP) in oral samples was highly correlated with serum CRP levels, suggesting that oral CRP levels may be used as a surrogate to predict serum CRP in AP patients. A multiplex cytokine/chemokine assay revealed that MCP-1 tended to be low, indicating unresponsiveness of MCP-1 and its downstream immune pathways in AP. Our findings suggest that oral salivary proteins, which are obtained non-invasively, can be utilized for the detection of AP.
Sections du résumé
BACKGROUND
BACKGROUND
Aspiration pneumonia (AP), which is a major cause of death in the elderly, does present with typical symptoms in the early stages of onset, thus it is difficult to detect and treat at an early stage. In this study, we identified biomarkers that are useful for the detection of AP and focused on salivary proteins, which may be collected non-invasively. Because expectorating saliva is often difficult for elderly people, we collected salivary proteins from the buccal mucosa.
METHODS
METHODS
We collected samples from the buccal mucosa of six patients with AP and six control patients (no AP) in an acute-care hospital. Following protein precipitation using trichloroacetic acid and washing with acetone, the samples were analyzed by liquid chromatography and tandem mass spectrometry (LC-MS/MS). We also determined the levels of cytokines and chemokines in non-precipitated samples from buccal mucosa.
RESULTS
RESULTS
Comparative quantitative analysis of LC-MS/MS spectra revealed 55 highly (P values < 0.10) abundant proteins with high FDR confidence (q values < 0.01) and high coverage (> 50%) in the AP group compared with the control group. Among the 55 proteins, the protein abundances of four proteins (protein S100-A7A, eukaryotic translation initiation factor 1, Serpin B4, and peptidoglycan recognition protein 1) in the AP group showed a negative correlation with the time post-onset; these proteins are promising AP biomarker candidates. In addition, the abundance of C-reactive protein (CRP) in oral samples was highly correlated with serum CRP levels, suggesting that oral CRP levels may be used as a surrogate to predict serum CRP in AP patients. A multiplex cytokine/chemokine assay revealed that MCP-1 tended to be low, indicating unresponsiveness of MCP-1 and its downstream immune pathways in AP.
CONCLUSION
CONCLUSIONS
Our findings suggest that oral salivary proteins, which are obtained non-invasively, can be utilized for the detection of AP.
Identifiants
pubmed: 36894881
doi: 10.1186/s12014-023-09398-w
pii: 10.1186/s12014-023-09398-w
pmc: PMC9996945
doi:
Types de publication
Journal Article
Langues
eng
Pagination
9Subventions
Organisme : Japan Society for the Promotion of Science,Japan
ID : 20H00560
Informations de copyright
© 2023. The Author(s).
Références
Gerodontology. 2017 Sep;34(3):343-356
pubmed: 28543778
Gene Expr Patterns. 2004 May;4(3):297-301
pubmed: 15053978
J Proteome Res. 2009 Jan;8(1):239-45
pubmed: 19118452
N Engl J Med. 2001 Mar 1;344(9):665-71
pubmed: 11228282
Proteomes. 2021 Mar 23;9(1):
pubmed: 33806881
RNA Biol. 2013 Aug;10(8):1291-8
pubmed: 23917022
Cytokine. 2017 Sep;97:108-116
pubmed: 28628889
Oncotarget. 2017 Dec 1;9(2):2895-2901
pubmed: 29416822
Immunol Lett. 2006 Feb 15;102(2):141-7
pubmed: 16214222
Annu Rev Physiol. 2016;78:481-504
pubmed: 26527186
N Engl J Med. 2019 Feb 14;380(7):651-663
pubmed: 30763196
PeerJ. 2018 Apr 5;6:e4557
pubmed: 29632742
PLoS One. 2014 Apr 09;9(4):e89366
pubmed: 24717448
Biomed Chromatogr. 2012 May;26(5):571-82
pubmed: 21898473
Int J Biochem Cell Biol. 2007;39(6):1165-76
pubmed: 17448722
Front Neurosci. 2021 May 26;15:668852
pubmed: 34121996
Nat Rev Rheumatol. 2015 Apr;11(4):202-3
pubmed: 25668140
Semin Cell Dev Biol. 2017 Feb;62:170-177
pubmed: 27637160
Am J Med. 2013 Nov;126(11):995-1001
pubmed: 24054176
Nat Genet. 2000 May;25(1):25-9
pubmed: 10802651
Nat Microbiol. 2016 Apr 04;1:16031
pubmed: 27572644
Brain Behav Immun. 2011 May;25(4):640-6
pubmed: 21236331
Nucleic Acids Res. 2021 Jan 8;49(D1):D325-D334
pubmed: 33290552
Aust Dent J. 2005 Sep;50(3):191-9
pubmed: 16238218
Adv Exp Med Biol. 2019;1073:23-56
pubmed: 31236838
J Dent Res. 2021 Dec;100(13):1510-1519
pubmed: 34032471
J Hosp Med. 2015 Feb;10(2):90-6
pubmed: 25363892
Clin Cancer Res. 2008 Oct 1;14(19):6246-52
pubmed: 18829504
J Interferon Cytokine Res. 2009 Jun;29(6):313-26
pubmed: 19441883
J Leukoc Biol. 1998 Jan;63(1):108-16
pubmed: 9469480
J Crit Care. 2015 Feb;30(1):40-8
pubmed: 25129577
Mol Neurobiol. 1997 Oct;15(2):131-63
pubmed: 9396008
J Bacteriol. 2010 Oct;192(19):5002-17
pubmed: 20656903
Database (Oxford). 2021 Oct 13;2021:
pubmed: 34642750
Am J Respir Cell Mol Biol. 2011 Feb;44(2):134-45
pubmed: 20299540
Nucleic Acids Res. 2017 Jan 4;45(D1):D1107-D1111
pubmed: 27899654
Mucosal Immunol. 2008 May;1(3):239-43
pubmed: 19079183
Front Immunol. 2018 Jan 05;8:1908
pubmed: 29379499
J Proteome Res. 2013 Apr 5;12(4):1700-9
pubmed: 23406527
J Biol Chem. 2006 Dec 15;281(50):38905-17
pubmed: 17030513
Respir Res. 2013 Nov 09;14:122
pubmed: 24209388
Arthritis Rheumatol. 2015 Apr;67(4):1084-95
pubmed: 25545990
J Rheumatol. 2008 Sep;35(9):1820-4
pubmed: 18634149
Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):1122-6
pubmed: 15657141