Plasma procalcitonin kinetics in healthy dogs and dogs undergoing tibial plateau leveling osteotomy.
canine
interindividual variability
intraindividual variability
orthopedic surgery
surgical site infection
Journal
Veterinary clinical pathology
ISSN: 1939-165X
Titre abrégé: Vet Clin Pathol
Pays: United States
ID NLM: 9880575
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
revised:
29
08
2022
received:
29
04
2022
accepted:
22
10
2022
medline:
6
6
2023
pubmed:
11
3
2023
entrez:
10
3
2023
Statut:
ppublish
Résumé
Procalcitonin (PCT) is a well-established biomarker for bacterial infection in human patients. We aimed to analyze the kinetics of plasma PCT (pPCT) in healthy dogs and dogs with canine cranial cruciate ligament (CCL) rupture undergoing tibial plateau leveling osteotomy (TPLO). This prospective, longitudinal study included 15 healthy dogs and 25 dogs undergoing TPLO. Hematology, pPCT, and C-reactive protein (CRP) were assessed on 3 consecutive days in healthy dogs and 1 day preoperatively and days 1, 2, 10, and 56 postoperatively. Inter- and intraindividual variability of pPCT were assessed in healthy dogs. Median pPCT concentrations of dogs with CCL rupture preoperatively were compared with healthy controls, and median pPCT concentrations, as well as percentage change post anesthesia, arthroscopy, and TPLO, were compared with baseline. For the correlation analysis, the Spearman rank correlation test was used. Inter- and intraindividual variabilities of pPCT in healthy dogs were 36% and 15%, respectively. Median baseline pPCT concentrations were not significantly different between healthy dogs (118.9 pg/mL; IQR: 75.3-157.3 pg/mL) and dogs undergoing TPLO (95.9 pg/mL; IQR: 63.8-117.0 pg/mL). Plasma PCT concentrations were significantly lower immediately post- than preoperatively (P < 0.001). CRP, WBC, and neutrophil concentrations increased significantly on post-OP day 2 and had normalized by day 10. These results indicate that CCL rupture, as well as anesthesia, arthroscopy, and TPLO combined, are not associated with increased pPCT concentrations in dogs with uncomplicated recovery. Considering the high intraindividual variability, individual serial measurements rather than a population-based reference interval should be considered.
Sections du résumé
BACKGROUND
BACKGROUND
Procalcitonin (PCT) is a well-established biomarker for bacterial infection in human patients.
OBJECTIVES
OBJECTIVE
We aimed to analyze the kinetics of plasma PCT (pPCT) in healthy dogs and dogs with canine cranial cruciate ligament (CCL) rupture undergoing tibial plateau leveling osteotomy (TPLO).
METHODS
METHODS
This prospective, longitudinal study included 15 healthy dogs and 25 dogs undergoing TPLO. Hematology, pPCT, and C-reactive protein (CRP) were assessed on 3 consecutive days in healthy dogs and 1 day preoperatively and days 1, 2, 10, and 56 postoperatively. Inter- and intraindividual variability of pPCT were assessed in healthy dogs. Median pPCT concentrations of dogs with CCL rupture preoperatively were compared with healthy controls, and median pPCT concentrations, as well as percentage change post anesthesia, arthroscopy, and TPLO, were compared with baseline. For the correlation analysis, the Spearman rank correlation test was used.
RESULTS
RESULTS
Inter- and intraindividual variabilities of pPCT in healthy dogs were 36% and 15%, respectively. Median baseline pPCT concentrations were not significantly different between healthy dogs (118.9 pg/mL; IQR: 75.3-157.3 pg/mL) and dogs undergoing TPLO (95.9 pg/mL; IQR: 63.8-117.0 pg/mL). Plasma PCT concentrations were significantly lower immediately post- than preoperatively (P < 0.001). CRP, WBC, and neutrophil concentrations increased significantly on post-OP day 2 and had normalized by day 10.
CONCLUSIONS
CONCLUSIONS
These results indicate that CCL rupture, as well as anesthesia, arthroscopy, and TPLO combined, are not associated with increased pPCT concentrations in dogs with uncomplicated recovery. Considering the high intraindividual variability, individual serial measurements rather than a population-based reference interval should be considered.
Substances chimiques
Procalcitonin
0
C-Reactive Protein
9007-41-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
360-368Subventions
Organisme : Swiss Federal Food Safety and Veterinary Office
ID : 1.20.03
Informations de copyright
© 2023 The Authors. Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of American Society for Veterinary Clinical Pathology.
Références
Deftos LJ, Roos BA, Parthemore JG. Calcium and skeletal metabolism. West J Med. 1975;123(6):447-458.
Assicot M, Bohuon C, Gendrel D, Raymond J, Carsin H, Guilbaud J. High serum procalcitonin concentrations in patients with sepsis and infection. Lancet. 1993;341:515-518. doi:10.1016/0140-6736(93)90277-N
Nijsten MWN, Olinga P, Hauw The T, et al. Procalcitonin behaves as a fast responding acute phase protein in vivo and in vitro. Crit Care Med. 2000;28(2):458-461. doi:10.1097/00003246-200002000-00028
Oberhoffer M, Stonans I, Russwurm S, et al. Procalcitonin expression in human peripheral blood mononuclear cells and its modulation by lipopolysaccharides and sepsis-related cytokines in vitro. J Lab Clin Med. 1999;134(1):49-55. doi:10.1016/S0022-2143(99)90053-7
Becker KL, Snider R, Nylen ES. Procalcitonin in sepsis and systemic inflammation: a harmful biomarker and a therapeutic target. Br J Pharmacol. 2010;159(2):253-264. doi:10.1111/j.1476-5381.2009.00433.x
Goggs R, Milloway M, Troia R, Giunti M. Plasma procalcitonin concentrations are increased in dogs with sepsis. Vet Rec. 2018;5:e000255. doi:10.1136/vetreco-2017-000255
Troia R, Giunti M, Goggs R. Plasma procalcitonin concentrations predict organ dysfunction and outcome in dogs with sepsis. BMC Vet Res. 2018;14:111. doi:10.1186/s12917-018-1427-y
Easley F, Holowaychuk MK, Lashnits EW, Nordone SK, Marr H, Birkenheuer AJ. Serum procalcitonin concentrations in dogs with induced endotoxemia. J Vet Intern Med. 2020;34:653-658. doi:10.1111/jvim.15711
Goggs R, Robbins SN, LaLonde-Paul DM, Menard JM. Serial analysis of blood biomarker concentrations in dogs with pneumonia, septic peritonitis, and pyometra. J Vet Intern Med. 2021;2022:1-16. doi:10.1111/jvim.16374
Vallet H, Chenevier-Gobeaux C, Villain C, et al. Prognostic value of serum procalcitonin after orthopedic surgery in the elderly population. J Gerontol. 2017;72(3):glw097. doi:10.1093/gerona/glw097
Ingber RB, Alhammoud A, Murray DP, et al. A systematic review and meta-analysis of procalcitonin as a marker of postoperative orthopedic infections. Orthopedics. 2018;41:e303-e309. doi:10.3928/01477447-20180409-07
Villain C, Chenevier-Gobeaux C, Cohen-Bittan J, et al. Procalcitonin and C-reactive protein for bacterial infection diagnosis in elderly patients after traumatic orthopedic surgery. J Gerontol. 2020;75(10):2008-2014. doi:10.1093/gerona/glz210
von Pfeil DJF, Kowaleski MP, Glassman M, Dejardin LM. Results of a survey of veterinary orthopedic society members on the preferred method for treating cranial cruciate ligament rupture in dogs weighing more than 15 kilograms (33 pounds). J Am Vet Med Assoc. 2018;253:586-597. doi:10.2460/javma.253.5.586
Pacchiana PD, Morris E, Gillings SL, Jessen CR, Lipowitz AJ. Surgical and postoperative complications associated with tibial plateau leveling osteotomy in dogs with cranial cruciate ligament rupture: 397 cases (1998-2001). J Am Vet Med Assoc. 2003;222:184-193. doi:10.2460/javma.2003.222.184
Bergh MS, Peirone B. Complications of tibial plateau levelling osteotomy in dogs. Vet Comp Orthop Traumatol. 2012;25:349-358. doi:10.3415/VCOT-11-09-0122
Coletti TJ, Anderson M, Gorse MJ, Madsen R. Complications associated with tibial plateau leveling osteotomy: a retrospective of 1519 procedures. Can Vet J. 2014;55(3):249-254.
National Healthcare Safety Network. Surgical site infection event (SSI). Centers Dis Control Prev. 2021.
Fitzpatrick N, Solano MA. Predictive variables for complications after TPLO with stifle inspection by arthrotomy in 1000 consecutive dogs. Vet Surg. 2010;39(4):460-474. doi:10.1111/j.1532-950X.2010.00663.x
Löfqvist K, Kjelgaard-Hansen M, Nielsen MBM. Usefulness of C-reactive protein and serum amyloid a in early detection of postoperative infectious complications to tibial plateau leveling osteotomy in dogs. Acta Vet Scand. 2018;60:30. doi:10.1186/s13028-018-0385-5
Neumann S, Steingräber L, Herold L. Investigation of procalcitonin and beta-defensin2 in the serum and feces of dogs with acute diarrhea. Vet Clin Pathol. 2022;50(S1):55-62. doi:10.1111/vcp.13099
Ahn S, Bae H, Kim J, et al. Comparison of clinical and inflammatory parameters in dogs with pyometra before and after ovariohysterectomy. Can J Vet Res. 2021;85(4):271-278.
Meisner M, Tschaikowsky K, Schnabel S, Schmidt J, Schüttler J, Katalinic A. Procalcitonin - influence of temperature, storage, anticoagulation and Arterialor venous Asservation of blood samples on procalcitonin concentrations. Clin Chem Lab Med. 1997;35(8):597-602. doi:10.1515/cclm.1997.35.8.597
Schuetz P, Christ-Crain M, Huber AR, Müller B. Long-term stability of procalcitonin in frozen samples and comparison of Kryptor® and VIDAS® automated immunoassays. Clin Biochem. 2010;43:341-344. doi:10.1016/j.clinbiochem.2009.08.029
Steinbach G, Rau B, Debard A-L, et al. Multicenter evaluation of a new immunoassay for procalcitonin measurement on the Kryptor. System. 2004;42. http://www.medcalc.be
Validation BM. Guidance for industry bioanalytical method validation. Vet Med. 2001..
Thomsson O, Ström-Holst B, Sjunnesson Y, Bergqvist AS. Validation of an enzyme-linked immunosorbent assay developed for measuring cortisol concentration in human saliva and serum for its applicability to analyze cortisol in pig saliva. Acta Vet Scand. 2014;56. doi:10.1186/s13028-014-0055-1
Hindenberg S, Klenner-Gastreich S, Kneier N, et al. Evaluation of a species-specific C-reactive protein assay for the dog on the ABX Pentra 400 clinical chemistry analyzer. BMC Vet Res. 2017;13(1):146. doi:10.1186/s12917-017-1065-9
Matomäki P, Kainulainen H, Kyröläinen H. Corrected whole blood biomarkers - the equation of dill and Costill revisited. Physiol Rep. 2018;6(12):e13749. doi:10.14814/phy2.13749
Canapp SO. The canine stifle. Clin Tech Small Anim Pract. 2007;22(4):195-205. doi:10.1053/j.ctsap.2007.09.008
Barassi A, Pallotti F, Melzi D'Eril GV. Biological variation of procalcitonin in healthy individuals [1]. Clin Chem. 2004;50:1878. doi:10.1373/clinchem.2004.037275
Aljabi Y, Manca A, Ryan J, Elshawarby A. Value of procalcitonin as a marker of surgical site infection following spinal surgery. Surgeon. 2019;17:97-101. doi:10.1016/j.surge.2018.05.006
Kjelgaard-Hansen M, Jacobsen S. Assay Validation and diagnostic applications of major acute-phase protein testing in companion animals. Clin Lab Med. 2011;31(1):51-70. doi:10.1016/j.cll.2010.10.002
Cerón JJ, Eckersall PD, Martínez-Subiela S. Acute phase proteins in dogs and cats: current knowledge and future perspectives. Vet Clin Pathol. 2005;34(2):85-99. doi:10.1111/j.1939-165X.2005.tb00019.x
Yasmin D, Bulut G, Yildiz M. Can procalcitonin be used for the diagnosis and follow-up of postoperative complications after fracture surgery? Acta Orthop Traumatol Turc. 2006;40(1):15-21.
Hagen CRM, Singh A, Weese JS, Marshall Q, Linden AZ, Gibson TW. Contributing factors to surgical site infection after tibial plateau leveling osteotomy: a follow-up retrospective study. Vet Surg. 2020;49:930-939. doi:10.1111/vsu.13436