Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy.
Journal
Light, science & applications
ISSN: 2047-7538
Titre abrégé: Light Sci Appl
Pays: England
ID NLM: 101610753
Informations de publication
Date de publication:
10 Mar 2023
10 Mar 2023
Historique:
received:
10
10
2022
accepted:
16
02
2023
revised:
07
02
2023
entrez:
10
3
2023
pubmed:
11
3
2023
medline:
11
3
2023
Statut:
epublish
Résumé
3D super-resolution microscopy with nanometric resolution is a key to fully complement ultrastructural techniques with fluorescence imaging. Here, we achieve 3D super-resolution by combining the 2D localization of pMINFLUX with the axial information of graphene energy transfer (GET) and the single-molecule switching by DNA-PAINT. We demonstrate <2 nm localization precision in all 3 dimension with axial precision reaching below 0.3 nm. In 3D DNA-PAINT measurements, structural features, i.e., individual docking strands at distances of 3 nm, are directly resolved on DNA origami structures. pMINFLUX and GET represent a particular synergetic combination for super-resolution imaging near the surface such as for cell adhesion and membrane complexes as the information of each photon is used for both 2D and axial localization information. Furthermore, we introduce local PAINT (L-PAINT), in which DNA-PAINT imager strands are equipped with an additional binding sequence for local upconcentration improving signal-to-background ratio and imaging speed of local clusters. L-PAINT is demonstrated by imaging a triangular structure with 6 nm side lengths within seconds.
Identifiants
pubmed: 36898993
doi: 10.1038/s41377-023-01111-8
pii: 10.1038/s41377-023-01111-8
pmc: PMC10006205
doi:
Types de publication
Journal Article
Langues
eng
Pagination
70Subventions
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : EXC 089/1-390776260
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 201269156-SFB1032
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : KA 5449/2-1
Informations de copyright
© 2023. The Author(s).
Références
Nat Methods. 2020 Aug;17(8):789-791
pubmed: 32601424
Nat Commun. 2021 Jul 21;12(1):4432
pubmed: 34290254
Angew Chem Int Ed Engl. 2021 Feb 23;60(9):4931-4938
pubmed: 33230933
Light Sci Appl. 2022 Jun 30;11(1):199
pubmed: 35773265
Nat Methods. 2015 Oct;12(10):935-8
pubmed: 26280329
Science. 2008 Jun 6;320(5881):1308
pubmed: 18388259
Nano Lett. 2010 Apr 14;10(4):1497-500
pubmed: 20218702
Nat Photonics. 2021 May;15(5):361-366
pubmed: 33953795
Nat Methods. 2019 Nov;16(11):1101-1104
pubmed: 31591576
Nano Lett. 2021 Jan 13;21(1):840-846
pubmed: 33336573
Nano Lett. 2019 Jul 10;19(7):4257-4262
pubmed: 31251640
Nano Lett. 2010 Nov 10;10(11):4756-61
pubmed: 20957983
Elife. 2021 Jan 29;10:
pubmed: 33513092
Cell. 2016 Aug 11;166(4):1028-1040
pubmed: 27397506
Nat Commun. 2021 Jan 22;12(1):517
pubmed: 33483489
Adv Mater. 2021 Oct;33(42):e2105719
pubmed: 34665480
Nat Commun. 2021 Feb 19;12(1):1180
pubmed: 33608524
Nat Methods. 2021 Apr;18(4):369-373
pubmed: 33795876
Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8206-10
pubmed: 10899992
Nat Methods. 2020 Feb;17(2):217-224
pubmed: 31932776
Science. 2008 Feb 8;319(5864):810-3
pubmed: 18174397
Science. 2016 Oct 21;354(6310):305-307
pubmed: 27846560
Nano Lett. 2018 Apr 11;18(4):2616-2622
pubmed: 29562123
J Chem Phys. 2008 Aug 7;129(5):054703
pubmed: 18698917
Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):2995-9
pubmed: 19211795
Nature. 2006 Mar 16;440(7082):297-302
pubmed: 16541064
Nat Methods. 2022 May;19(5):554-559
pubmed: 35501386
ACS Nano. 2021 Apr 27;15(4):6430-6438
pubmed: 33834769
J Am Chem Soc. 2009 Apr 15;131(14):5018-9
pubmed: 19301868
Science. 2017 Feb 10;355(6325):606-612
pubmed: 28008086
Sci Adv. 2022 Jun 10;8(23):eabo2506
pubmed: 35675401