Neurofilament-light chain quantification by Simoa and Ella in plasma from patients with dementia: a comparative study.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
10 03 2023
10 03 2023
Historique:
received:
01
09
2022
accepted:
09
02
2023
entrez:
10
3
2023
pubmed:
11
3
2023
medline:
15
3
2023
Statut:
epublish
Résumé
Neurofilament light chains (NfL) are neuron-specific cytoskeletal proteins whose plasmatic concentrations have been explored as a clinically useful marker in several types of dementia. Plasma concentrations of NfL are extremely low, and just two assays are commercially available for their study: one based on the SiMoA technology and one based on Ella. We thus studied plasma levels of NfL with both platforms to check the correlation between them and to assess their potential in the diagnosis of neurodegeneration. Plasma NfL levels were measured on 50 subjects: 18 healthy controls, 20 Alzheimer's disease, and 12 frontotemporal dementia patients. Ella returned plasmatic NfL levels significantly higher than SiMoA, however the results were strongly correlated (r = 0.94), and a proportional coefficient of 0.58 between the two assays was calculated. Both assays detected higher plasma NfL levels in patients with dementia than in the control group (p < 0.0001) and allowed their discrimination with excellent diagnostic performance (AUC > 0.95). No difference was found between Alzheimer's and Frontotemporal dementia either using SiMoA or Ella. In conclusion, both the analytical platforms resulted effective in analysing plasma levels of NfL. However, the correct interpretation of results requires the precise knowledge of the assay used.
Identifiants
pubmed: 36899015
doi: 10.1038/s41598-023-29704-8
pii: 10.1038/s41598-023-29704-8
pmc: PMC10006166
doi:
Substances chimiques
Biomarkers
0
Neurofilament Proteins
0
Cytoskeletal Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4041Informations de copyright
© 2023. The Author(s).
Références
Brain. 2011 Sep;134(Pt 9):2456-77
pubmed: 21810890
Lancet Neurol. 2022 Jan;21(1):66-77
pubmed: 34838239
Mult Scler. 2022 Sep;28(10):1553-1561
pubmed: 35282740
J Immunol Methods. 2017 Dec;451:1-10
pubmed: 28917434
J Neurol Neurosurg Psychiatry. 2022 Jun;93(6):651-658
pubmed: 35078917
Nat Biotechnol. 2010 Jun;28(6):595-9
pubmed: 20495550
Bioanalysis. 2018 Feb;10(3):181-194
pubmed: 29345151
Alzheimers Dement. 2011 May;7(3):263-9
pubmed: 21514250
Nat Med. 2018 May;24(5):598-603
pubmed: 29736028
Anal Chim Acta. 2018 Dec 11;1037:293-300
pubmed: 30292305
Lancet Neurol. 2022 Mar;21(3):246-257
pubmed: 35182510
Clin Chem Lab Med. 2016 Oct 1;54(10):1655-61
pubmed: 27071153
Brain. 2022 Jun 3;145(5):1598-1609
pubmed: 35202463
Neurosci Biobehav Rev. 2019 Jul;102:123-138
pubmed: 31026486
Neurobiol Aging. 2020 Oct;94:60-70
pubmed: 32585491
J Alzheimers Dis. 2022;87(1):131-140
pubmed: 35275542
J Alzheimers Dis. 2022;85(3):1021-1029
pubmed: 34924380
J Neurol. 2022 Aug;269(8):4270-4280
pubmed: 35288777
BMC Neurol. 2022 Jan 27;22(1):39
pubmed: 35086487
PLoS One. 2013 Sep 20;8(9):e75091
pubmed: 24073237
Neurology. 2022 Mar 15;98(11):e1137-e1150
pubmed: 35173015
Nat Commun. 2021 Jun 7;12(1):3400
pubmed: 34099648
Nat Rev Neurol. 2018 Oct;14(10):577-589
pubmed: 30171200
Neurology. 2021 May 4;96(18):e2296-e2312
pubmed: 33827960
Transl Neurodegener. 2021 Aug 26;10(1):31
pubmed: 34433481
Sci Rep. 2020 Jun 8;10(1):9161
pubmed: 32514050
Alzheimers Dement (Amst). 2021 Jun 02;13(1):e12168
pubmed: 34124336
Brain. 2023 Feb 13;146(2):690-699
pubmed: 35383826
Aging Clin Exp Res. 2022 Feb;34(2):331-339
pubmed: 35018623
J Neurol Neurosurg Psychiatry. 2019 Aug;90(8):870-881
pubmed: 30967444
Ann Clin Transl Neurol. 2021 May;8(5):1141-1150
pubmed: 33830650
J Neurol Neurosurg Psychiatry. 2015 Mar;86(3):273-9
pubmed: 24935984
J Neuroimmunol. 2021 Sep 15;358:577662
pubmed: 34311152
Alzheimers Res Ther. 2021 May 5;13(1):95
pubmed: 33952308
Nat Rev Neurol. 2019 Oct;15(10):557
pubmed: 31506590
Ann Neurol. 2017 Jun;81(6):857-870
pubmed: 28512753