Endometrial Injury Upregulates Expression of Receptivity Genes in Women with Implantation Failure.
endometrium
fertility outcome
homeobox gene
implantation failure
mRNA
protein
Journal
International journal of environmental research and public health
ISSN: 1660-4601
Titre abrégé: Int J Environ Res Public Health
Pays: Switzerland
ID NLM: 101238455
Informations de publication
Date de publication:
23 02 2023
23 02 2023
Historique:
received:
03
12
2022
revised:
13
02
2023
accepted:
16
02
2023
entrez:
11
3
2023
pubmed:
12
3
2023
medline:
15
3
2023
Statut:
epublish
Résumé
Homeobox genes A10 (HOXA10) and A11 (HOXA11), members of the abdominal B gene family, are responsible for embryonic survival and implantation. This study was planned to investigate whether endometrial injury alters the expression of both transcripts in women with implantation failure. A total of 54 women with implantation failure were divided into two equal groups as experimental (scratching) and sham (no scratching). Participants in the scratching group were exposed to endometrial injury in the mid-luteal phase, and those in the sham group were exposed to endometrial flushing. The scratching group, but not the sham group, underwent prior endometrial sampling. A second endometrial sampling was performed on the scratching group in the mid-luteal phase of the following cycle. The mRNA and protein levels of the HOXA10 and 11 transcripts were determined in endometrial samples collected before and after injury/flushing. Participants in each group underwent IVF/ET in the cycle after the second endometrial sampling. Endometrial injury caused a 60.1-fold ( Endometrial injury increases homeobox transcript expression at both mRNA and protein levels.
Sections du résumé
BACKGROUND
Homeobox genes A10 (HOXA10) and A11 (HOXA11), members of the abdominal B gene family, are responsible for embryonic survival and implantation. This study was planned to investigate whether endometrial injury alters the expression of both transcripts in women with implantation failure.
METHODS
A total of 54 women with implantation failure were divided into two equal groups as experimental (scratching) and sham (no scratching). Participants in the scratching group were exposed to endometrial injury in the mid-luteal phase, and those in the sham group were exposed to endometrial flushing. The scratching group, but not the sham group, underwent prior endometrial sampling. A second endometrial sampling was performed on the scratching group in the mid-luteal phase of the following cycle. The mRNA and protein levels of the HOXA10 and 11 transcripts were determined in endometrial samples collected before and after injury/flushing. Participants in each group underwent IVF/ET in the cycle after the second endometrial sampling.
RESULTS
Endometrial injury caused a 60.1-fold (
CONCLUSIONS
Endometrial injury increases homeobox transcript expression at both mRNA and protein levels.
Identifiants
pubmed: 36900953
pii: ijerph20053942
doi: 10.3390/ijerph20053942
pmc: PMC10002420
pii:
doi:
Substances chimiques
Transcription Factors
0
HOXA10 protein, human
140441-81-2
HOXA11 protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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