Small Cellular Particles from European Spruce Needle Homogenate.
Picea abies
cell-to-cell communication
drug delivery
exosomes
extracellular particles
extracellular vesicles
interferometric light microscopy
small cellular particles
spruce needles
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
22 Feb 2023
22 Feb 2023
Historique:
received:
20
12
2022
revised:
17
02
2023
accepted:
18
02
2023
entrez:
11
3
2023
pubmed:
12
3
2023
medline:
15
3
2023
Statut:
epublish
Résumé
Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. SCPs were isolated by differential ultracentrifugation. They were imaged by scanning electron microscope (SEM) and cryogenic transmission electron microscope (cryo TEM), assessed for their number density and hydrodynamic diameter by interferometric light microscopy (ILM) and flow cytometry (FCM), total phenolic content (TPC) by UV-vis spectroscopy, and terpene content by gas chromatography-mass spectrometry (GC-MS). The supernatant after ultracentrifugation at 50,000×
Identifiants
pubmed: 36901780
pii: ijms24054349
doi: 10.3390/ijms24054349
pmc: PMC10001569
pii:
doi:
Substances chimiques
Terpenes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Slovenian Research Agency
ID : P2-0232, P3-0388, P1-0201, P1-0391, J3-3066, L3-2621, J2-4447, IO-0003
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