Peroxisomes Are Highly Abundant and Heterogeneous in Human Parotid Glands.
PSP
catalase
differential expression
human
immunofluorescence
mRNA
parotid gland
peroxisomes
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
01 Mar 2023
01 Mar 2023
Historique:
received:
29
12
2022
revised:
18
02
2023
accepted:
20
02
2023
entrez:
11
3
2023
pubmed:
12
3
2023
medline:
15
3
2023
Statut:
epublish
Résumé
The parotid gland is one of the major salivary glands producing a serous secretion, and it plays an essential role in the digestive and immune systems. Knowledge of peroxisomes in the human parotid gland is minimal; furthermore, the peroxisomal compartment and its enzyme composition in the different cell types of the human parotid gland have never been subjected to a detailed investigation. Therefore, we performed a comprehensive analysis of peroxisomes in the human parotid gland's striated duct and acinar cells. We combined biochemical techniques with various light and electron microscopy techniques to determine the localization of parotid secretory proteins and different peroxisomal marker proteins in parotid gland tissue. Moreover, we analyzed the mRNA of numerous gene encoding proteins localized in peroxisomes using real-time quantitative PCR. The results confirm the presence of peroxisomes in all striated duct and acinar cells of the human parotid gland. Immunofluorescence analyses for various peroxisomal proteins showed a higher abundance and more intense staining in striated duct cells compared to acinar cells. Moreover, human parotid glands comprise high quantities of catalase and other antioxidative enzymes in discrete subcellular regions, suggesting their role in protection against oxidative stress. This study provides the first thorough description of parotid peroxisomes in different parotid cell types of healthy human tissue.
Identifiants
pubmed: 36902220
pii: ijms24054783
doi: 10.3390/ijms24054783
pmc: PMC10003153
pii:
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : University of Giessen
ID : Leistungsorientierte Mittel
Références
Biotechniques. 2016 Apr 01;60(4):200-3
pubmed: 27071609
Biomed Res Int. 2018 Jan 30;2018:4340871
pubmed: 29651432
Prog Lipid Res. 2011 Jan;50(1):35-51
pubmed: 20655950
Anat Rec. 1988 Mar;220(3):239-51
pubmed: 3284416
J Proteome Res. 2006 Jun;5(6):1493-503
pubmed: 16740002
J Histochem Cytochem. 1973 Feb;21(2):131-41
pubmed: 4121048
Future Cardiol. 2017 May;13(3):279-296
pubmed: 28581362
Radiat Res. 2003 Apr;159(4):465-70
pubmed: 12643791
J Anat. 1976 Dec;122(Pt 3):627-40
pubmed: 1010791
Physiol Rev. 2023 Jan 1;103(1):957-1024
pubmed: 35951481
Histochem J. 1977 Nov;9(6):711-28
pubmed: 924808
Histochem Cell Biol. 2009 Sep;132(3):339-49
pubmed: 19499239
J Clin Biochem Nutr. 2016 Sep;59(2):79-85
pubmed: 27698533
Biomed Res Int. 2016;2016:6786245
pubmed: 27766264
Histochem Cell Biol. 2013 Oct;140(4):423-42
pubmed: 23959168
J Clin Biochem Nutr. 2017 Jul;61(1):40-46
pubmed: 28751808
Mol Cell Biol. 2002 Jun;22(12):4358-65
pubmed: 12024045
Gen Pharmacol. 1980;11(6):507-11
pubmed: 7461427
Histochem J. 1983 Jun;15(6):543-55
pubmed: 6409845
Mol Cell Biol. 2004 Feb;24(3):1313-23
pubmed: 14729975
Arch Oral Biol. 1986;31(2):87-93
pubmed: 3460541
J Cell Biol. 2002 Feb 18;156(4):643-51
pubmed: 11839773
Annu Rev Biochem. 2006;75:295-332
pubmed: 16756494
J Cell Biol. 1981 Aug;90(2):533-6
pubmed: 7026575
Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13748-53
pubmed: 8943006
Int J Mol Sci. 2021 Jul 23;22(15):
pubmed: 34360635
Arch Biochem Biophys. 1991 Feb 1;284(2):326-31
pubmed: 1989516
Int J Mol Sci. 2019 Aug 08;20(16):
pubmed: 31398943
Int J Clin Exp Pathol. 2014 Jul 15;7(8):4886-94
pubmed: 25197359
Anat Rec. 1986 Feb;214(2):165-7
pubmed: 3954071
Oral Dis. 2016 Jan;22(1):9-18
pubmed: 25417961
Stain Technol. 1977 Jul;52(4):238-9
pubmed: 333657
Prog Lipid Res. 2002 Sep;41(5):369-91
pubmed: 12121718
Mol Cell Biol. 2002 Dec;22(23):8226-40
pubmed: 12417726
J Anat. 1978 Sep;127(Pt 1):181-91
pubmed: 701194
J Biol Chem. 2011 Jul 15;286(28):24916-30
pubmed: 21515882
Histochem Cell Biol. 2008 Oct;130(4):719-40
pubmed: 18665385
Nature. 1990 Oct 18;347(6294):645-50
pubmed: 2129546
Front Endocrinol (Lausanne). 2021 Feb 26;12:624112
pubmed: 33716977
J Histochem Cytochem. 1966 Apr;14(4):291-302
pubmed: 5962951
Biochimie. 2014 Mar;98:75-85
pubmed: 24211592
Histochemistry. 1981;71(1):33-44
pubmed: 6262282
Proc Natl Acad Sci U S A. 2000 May 23;97(11):5746-9
pubmed: 10811907
Annu Rev Food Sci Technol. 2013;4:267-76
pubmed: 23464573
PLoS One. 2013 May 02;8(5):e63062
pubmed: 23658797
J Dent Res. 2011 Sep;90(9):1085-90
pubmed: 21628641
Histochem Cell Biol. 1997 Sep;108(3):185-210
pubmed: 9342614
Anat Rec. 1998 May;251(1):122-35
pubmed: 9605228
J Comp Neurol. 2007 Nov 1;505(1):1-17
pubmed: 17729295
Biol Reprod. 2007 Dec;77(6):1060-72
pubmed: 17881773
Proteomes. 2013 Dec 1;1(3):275-289
pubmed: 24926433
Biochim Biophys Acta. 2006 Sep;1761(9):973-94
pubmed: 16766224
J Clin Biochem Nutr. 2015 Jan;56(1):1-7
pubmed: 25834301
J Biol Chem. 1990 Apr 5;265(10):5731-5
pubmed: 1969406
J Proteome Res. 2008 May;7(5):1994-2006
pubmed: 18361515
Cell Tissue Res. 2016 Jun;364(3):543-557
pubmed: 26796206
Histochem Cell Biol. 2009 Apr;131(4):447-54
pubmed: 19229552