Effect of Chitosan-Diosgenin Combination on Wound Healing.
chitosan
diosgenin
murine excisional wound model
oxidative stress
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
06 Mar 2023
06 Mar 2023
Historique:
received:
30
01
2023
revised:
03
03
2023
accepted:
05
03
2023
entrez:
11
3
2023
pubmed:
12
3
2023
medline:
15
3
2023
Statut:
epublish
Résumé
The difficult-to-heal wounds continue to be a problem for modern medicine. Chitosan and diosgenin possess anti-inflammatory and antioxidant effects making them relevant substances for wound treatment. That is why this work aimed to study the effect of the combined application of chitosan and diosgenin on a mouse skin wound model. For the purpose, wounds (6 mm diameter) were made on mice's backs and were treated for 9 days with one of the following: 50% ethanol (control), polyethylene glycol (PEG) in 50% ethanol, chitosan and PEG in 50% ethanol (Chs), diosgenin and PEG in 50% ethanol (Dg) and chitosan, diosgenin and PEG in 50% ethanol (ChsDg). Before the first treatment and on the 3rd, 6th and 9th days, the wounds were photographed and their area was determined. On the 9th day, animals were euthanized and wounds' tissues were excised for histological analysis. In addition, the lipid peroxidation (LPO), protein oxidation (POx) and total glutathione (tGSH) levels were measured. The results showed that ChsDg had the most pronounced overall effect on wound area reduction, followed by Chs and PEG. Moreover, the application of ChsDg maintained high levels of tGSH in wound tissues, compared to other substances. It was shown that all tested substances, except ethanol, reduced POx comparable to intact skin levels. Therefore, the combined application of chitosan and diosgenin is a very promising and effective medication for wound healing.
Identifiants
pubmed: 36902475
pii: ijms24055049
doi: 10.3390/ijms24055049
pmc: PMC10003508
pii:
doi:
Substances chimiques
Chitosan
9012-76-4
Diosgenin
K49P2K8WLX
Antioxidants
0
Glutathione
GAN16C9B8O
Ethanol
3K9958V90M
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Education and Science
ID : Grant D01-217/30.11.2018
Références
Int J Biomater. 2021 Oct 14;2021:4877344
pubmed: 34691184
Wound Repair Regen. 2002 Jan-Feb;10(1):52-8
pubmed: 11983006
J Control Release. 2022 Nov;351:215-230
pubmed: 36165835
Front Physiol. 2017 Jun 27;8:428
pubmed: 28701960
Oxid Med Cell Longev. 2022 May 29;2022:1035441
pubmed: 35677108
Physiol Rev. 1998 Apr;78(2):547-81
pubmed: 9562038
Microsc Res Tech. 1998 Sep 1;42(5):345-50
pubmed: 9766429
Antioxidants (Basel). 2018 Jul 24;7(8):
pubmed: 30042332
Cancer Lett. 2011 Feb 28;301(2):193-202
pubmed: 21195543
Adv Drug Deliv Rev. 2001 Nov 5;52(2):105-15
pubmed: 11718934
Wound Repair Regen. 2015 Nov-Dec;23(6):874-7
pubmed: 26136050
J Clin Med. 2021 Aug 13;10(16):
pubmed: 34441854
Gels. 2018 Aug 09;4(3):
pubmed: 30674843
Front Bioeng Biotechnol. 2021 Feb 18;9:650598
pubmed: 33681176
Future Sci OA. 2017 Sep 14;4(1):FSO225
pubmed: 29255618
Biomedicine (Taipei). 2015 Dec;5(4):22
pubmed: 26615539
J Pharm Pharmacol. 2022 Apr 20;74(4):485-502
pubmed: 33822141
Int J Mol Med. 2019 Jul;44(1):3-15
pubmed: 31115493
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 May 15;877(14-15):1530-6
pubmed: 19372064
Hepatology. 1997 Feb;25(2):361-7
pubmed: 9021948
Arch Dermatol Res. 2016 Aug;308(6):443-8
pubmed: 27262586
J Int Med Res. 2009 Sep-Oct;37(5):1528-42
pubmed: 19930861
Biomaterials. 2001 Jan;22(2):165-73
pubmed: 11101160
Biotechnol Adv. 2011 May-Jun;29(3):322-37
pubmed: 21262336
Phytochemistry. 2007 Mar;68(5):623-30
pubmed: 17166529
Nat Prod Res. 2016 Aug;30(16):1830-3
pubmed: 26222585
J Trace Elem Med Biol. 2020 Mar;58:126426
pubmed: 31743802
Biomaterials. 2002 Jul;23(13):2641-8
pubmed: 12059013
Dermatol Surg. 2008 Sep;34(9):1159-69
pubmed: 18513296
Inflamm Regen. 2021 Feb 2;41(1):4
pubmed: 33526070
Curr Probl Surg. 2001 Feb;38(2):72-140
pubmed: 11452260
Mater Sci Eng C Mater Biol Appl. 2018 Aug 1;89:213-222
pubmed: 29752091
J Pharm Sci. 2015 Nov;104(11):3653-3680
pubmed: 26308473
Scand J Plast Reconstr Surg. 1984;18(1):127-33
pubmed: 6377482
Food Chem. 2013 Nov 15;141(2):1104-13
pubmed: 23790892
J Ethnopharmacol. 2021 Apr 24;270:113842
pubmed: 33460752
J Clin Med. 2019 Dec 26;9(1):
pubmed: 31887972
Nat Protoc. 2006;1(6):3159-65
pubmed: 17406579
J Am Acad Orthop Surg. 2018 Dec 1;26(23):833-843
pubmed: 30180089
Chin J Nat Med. 2015 Aug;13(8):578-87
pubmed: 26253490
Int J Biol Macromol. 2021 Aug 1;184:701-712
pubmed: 34157330
Biomaterials. 2006 Aug;27(22):4157-64
pubmed: 16616364
J Immunol. 2002 Mar 1;168(5):2560-7
pubmed: 11859152
Clin Chim Acta. 2003 Mar;329(1-2):23-38
pubmed: 12589963
Drug Metab Rev. 2000 Aug-Nov;32(3-4):307-26
pubmed: 11139131
Int J Exp Pathol. 2000 Aug;81(4):257-63
pubmed: 10971747
Biomaterials. 2009 Feb;30(4):436-44
pubmed: 18977028
Nat Prod Res. 2012;26(23):2243-6
pubmed: 22235932
Oxid Med Cell Longev. 2014;2014:360438
pubmed: 24999379
Redox Biol. 2014;2:99-104
pubmed: 25460724
Lipids Health Dis. 2011 May 16;10:78
pubmed: 21575256