In Vitro Generation of Human Cross-Presenting Type 1 Conventional Dendritic Cells (cDC1s) and Plasmacytoid Dendritic Cells (pDCs).
CD34+ hematopoietic stem cells
Hematopoiesis
Notch
Plasmacytoid dendritic cells
Stromal cells
Type 1 conventional dendritic cells
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
11
3
2023
pubmed:
12
3
2023
medline:
15
3
2023
Statut:
ppublish
Résumé
Dendritic cells (DCs) represent one of the most important immune cell subsets in preventing the host from pathogen invasion by promoting both innate and adaptive immunity. Most research on human dendritic cells has focused on the easy-to-obtain dendritic cells derived in vitro from monocytes (MoDCs). However, many questions remain unanswered regarding the role of different dendritic cell types. The investigation of their roles in human immunity is hampered by their rarity and fragility, which especially holds true for type 1 conventional dendritic cells (cDC1s) and for plasmacytoid dendritic cells (pDCs). In vitro differentiation from hematopoietic progenitors emerged as a common way to produce different DC types, but the efficiency and reproducibility of these protocols needed to be improved and the extent to which the DCs generated in vitro resembled their in vivo counterparts required a more rigorous and global assessment. Here, we describe a cost-effective and robust in vitro differentiation system for the production of cDC1s and pDCs equivalent to their blood counterparts, from cord blood CD34
Identifiants
pubmed: 36905514
doi: 10.1007/978-1-0716-2938-3_10
doi:
Substances chimiques
Antigens, CD34
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
133-145Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Mildner A, Jung S (2014) Development and function of dendritic cell subsets. Immunity 40(5):642–656. https://doi.org/10.1016/j.immuni.2014.04.016
doi: 10.1016/j.immuni.2014.04.016
pubmed: 24837101
Dzionek A, Fuchs A, Schmidt P, Cremer S, Zysk M, Miltenyi S, Buck DW, Schmitz J (2000) BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol 165(11):6037–6046. https://doi.org/10.4049/jimmunol.165.11.6037
doi: 10.4049/jimmunol.165.11.6037
pubmed: 11086035
Vu Manh TP, Bertho N, Hosmalin A, Schwartz-Cornil I, Dalod M (2015) Investigating evolutionary conservation of dendritic cell subset identity and functions. Front Immunol 6:260. https://doi.org/10.3389/fimmu.2015.00260
doi: 10.3389/fimmu.2015.00260
pubmed: 26082777
pmcid: 4451681
Jongbloed SL, Kassianos AJ, McDonald KJ, Clark GJ, Ju X, Angel CE, Chen CJ, Dunbar PR, Wadley RB, Jeet V, Vulink AJ, Hart DN, Radford KJ (2010) Human CD141+ (BDCA-3)+ dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens. J Exp Med 207(6):1247–1260. https://doi.org/10.1084/jem.20092140
doi: 10.1084/jem.20092140
pubmed: 20479116
pmcid: 2882828
Bachem A, Guttler S, Hartung E, Ebstein F, Schaefer M, Tannert A, Salama A, Movassaghi K, Opitz C, Mages HW, Henn V, Kloetzel PM, Gurka S, Kroczek RA (2010) Superior antigen cross-presentation and XCR1 expression define human CD11c+CD141+ cells as homologues of mouse CD8+ dendritic cells. J Exp Med 207(6):1273–1281. https://doi.org/10.1084/jem.20100348
doi: 10.1084/jem.20100348
pubmed: 20479115
pmcid: 2882837
Crozat K, Guiton R, Contreras V, Feuillet V, Dutertre CA, Ventre E, Vu Manh TP, Baranek T, Storset AK, Marvel J, Boudinot P, Hosmalin A, Schwartz-Cornil I, Dalod M (2010) The XC chemokine receptor 1 is a conserved selective marker of mammalian cells homologous to mouse CD8alpha+ dendritic cells. J Exp Med 207(6):1283–1292. https://doi.org/10.1084/jem.20100223
doi: 10.1084/jem.20100223
pubmed: 20479118
pmcid: 2882835
Heger L, Balk S, Luhr JJ, Heidkamp GF, Lehmann CHK, Hatscher L, Purbojo A, Hartmann A, Garcia-Martin F, Nishimura SI, Cesnjevar R, Nimmerjahn F, Dudziak D (2018) CLEC10A is a specific marker for human CD1c(+) dendritic cells and enhances their toll-like receptor 7/8-induced cytokine secretion. Front Immunol 9:744. https://doi.org/10.3389/fimmu.2018.00744
doi: 10.3389/fimmu.2018.00744
pubmed: 29755453
pmcid: 5934495
Heger L, Hofer TP, Bigley V, de Vries IJM, Dalod M, Dudziak D, Ziegler-Heitbrock L (2020) Subsets of CD1c(+) DCs: dendritic cell versus monocyte lineage. Front Immunol 11:559166. https://doi.org/10.3389/fimmu.2020.559166
doi: 10.3389/fimmu.2020.559166
pubmed: 33101275
pmcid: 7554627
Tomasello E, Pollet E, Vu Manh TP, Uze G, Dalod M (2014) Harnessing mechanistic knowledge on beneficial versus deleterious IFN-I effects to design innovative immunotherapies targeting cytokine activity to specific cell types. Front Immunol 5:526. https://doi.org/10.3389/fimmu.2014.00526
doi: 10.3389/fimmu.2014.00526
pubmed: 25400632
pmcid: 4214202
Schoggins JW (2019) Interferon-stimulated genes: what do they all do? Annu Rev Virol 6(1):567–584. https://doi.org/10.1146/annurev-virology-092818-015756
doi: 10.1146/annurev-virology-092818-015756
pubmed: 31283436
Cabeza-Cabrerizo M, Cardoso A, Minutti CM, Pereira da Costa M, Reis ESC (2021) Dendritic cells revisited. Annu Rev Immunol 39:131. https://doi.org/10.1146/annurev-immunol-061020-053707
doi: 10.1146/annurev-immunol-061020-053707
pubmed: 33481643
Chen XQ, Liu XF, Liu WH, Guo W, Yu Q, Wang CY (2013) Comparative analysis of dendritic cell numbers and subsets between smoking and control subjects in the peripheral blood. Int J Clin Exp Pathol 6(2):290–296
pubmed: 23330015
pmcid: 3544240
Sallusto F, Lanzavecchia A (1994) Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha. J Exp Med 179(4):1109–1118. https://doi.org/10.1084/jem.179.4.1109
doi: 10.1084/jem.179.4.1109
pubmed: 8145033
Tacken PJ, de Vries IJ, Torensma R, Figdor CG (2007) Dendritic-cell immunotherapy: from ex vivo loading to in vivo targeting. Nat Rev Immunol 7(10):790–802. https://doi.org/10.1038/nri2173
doi: 10.1038/nri2173
pubmed: 17853902
Crozat K, Guiton R, Guilliams M, Henri S, Baranek T, Schwartz-Cornil I, Malissen B, Dalod M (2010) Comparative genomics as a tool to reveal functional equivalences between human and mouse dendritic cell subsets. Immunol Rev 234(1):177–198. https://doi.org/10.1111/j.0105-2896.2009.00868.x
doi: 10.1111/j.0105-2896.2009.00868.x
pubmed: 20193019
Robbins SH, Walzer T, Dembele D, Thibault C, Defays A, Bessou G, Xu H, Vivier E, Sellars M, Pierre P, Sharp FR, Chan S, Kastner P, Dalod M (2008) Novel insights into the relationships between dendritic cell subsets in human and mouse revealed by genome-wide expression profiling. Genome Biol 9(1):R17. https://doi.org/10.1186/gb-2008-9-1-r17
doi: 10.1186/gb-2008-9-1-r17
pubmed: 18218067
pmcid: 2395256
Chaperot L, Bendriss N, Manches O, Gressin R, Maynadie M, Trimoreau F, Orfeuvre H, Corront B, Feuillard J, Sotto JJ, Bensa JC, Briere F, Plumas J, Jacob MC (2001) Identification of a leukemic counterpart of the plasmacytoid dendritic cells. Blood 97(10):3210–3217. https://doi.org/10.1182/blood.v97.10.3210
doi: 10.1182/blood.v97.10.3210
pubmed: 11342451
Chaperot L, Perrot I, Jacob MC, Blanchard D, Salaun V, Deneys V, Lebecque S, Briere F, Bensa JC, Plumas J (2004) Leukemic plasmacytoid dendritic cells share phenotypic and functional features with their normal counterparts. Eur J Immunol 34(2):418–426. https://doi.org/10.1002/eji.200324531
doi: 10.1002/eji.200324531
pubmed: 14768046
Maeda T, Murata K, Fukushima T, Sugahara K, Tsuruda K, Anami M, Onimaru Y, Tsukasaki K, Tomonaga M, Moriuchi R, Hasegawa H, Yamada Y, Kamihira S (2005) A novel plasmacytoid dendritic cell line, CAL-1, established from a patient with blastic natural killer cell lymphoma. Int J Hematol 81(2):148–154. https://doi.org/10.1532/ijh97.04116
doi: 10.1532/ijh97.04116
pubmed: 15765784
Steinhagen F, McFarland AP, Rodriguez LG, Tewary P, Jarret A, Savan R, Klinman DM (2013) IRF-5 and NF-kappaB p50 co-regulate IFN-beta and IL-6 expression in TLR9-stimulated human plasmacytoid dendritic cells. Eur J Immunol 43(7):1896–1906. https://doi.org/10.1002/eji.201242792
doi: 10.1002/eji.201242792
pubmed: 23616277
pmcid: 6389267
Steinhagen F, Rodriguez LG, Tross D, Tewary P, Bode C, Klinman DM (2016) IRF5 and IRF8 modulate the CAL-1 human plasmacytoid dendritic cell line response following TLR9 ligation. Eur J Immunol 46(3):647–655. https://doi.org/10.1002/eji.201545911
doi: 10.1002/eji.201545911
pubmed: 26613957
Pelka K, Latz E (2013) IRF5, IRF8, and IRF7 in human pDCs – the good, the bad, and the insignificant? Eur J Immunol 43(7):1693–1697. https://doi.org/10.1002/eji.201343739
doi: 10.1002/eji.201343739
pubmed: 23828296
Ciancanelli MJ, Huang SX, Luthra P, Garner H, Itan Y, Volpi S, Lafaille FG, Trouillet C, Schmolke M, Albrecht RA, Israelsson E, Lim HK, Casadio M, Hermesh T, Lorenzo L, Leung LW, Pedergnana V, Boisson B, Okada S, Picard C, Ringuier B, Troussier F, Chaussabel D, Abel L, Pellier I, Notarangelo LD, Garcia-Sastre A, Basler CF, Geissmann F, Zhang SY, Snoeck HW, Casanova JL (2015) Infectious disease. Life-threatening influenza and impaired interferon amplification in human IRF7 deficiency. Science 348(6233):448–453. https://doi.org/10.1126/science.aaa1578
doi: 10.1126/science.aaa1578
pubmed: 25814066
pmcid: 4431581
Balan S, Arnold-Schrauf C, Abbas A, Couespel N, Savoret J, Imperatore F, Villani AC, Vu Manh TP, Bhardwaj N, Dalod M (2018) Large-scale human dendritic cell differentiation revealing notch-dependent lineage bifurcation and heterogeneity. Cell Rep 24(7):1902–1915. e1906. https://doi.org/10.1016/j.celrep.2018.07.033
doi: 10.1016/j.celrep.2018.07.033
pubmed: 30110645
pmcid: 6113934
Silvin A, Yu CI, Lahaye X, Imperatore F, Brault JB, Cardinaud S, Becker C, Kwan WH, Conrad C, Maurin M, Goudot C, Marques-Ladeira S, Wang Y, Pascual V, Anguiano E, Albrecht RA, Iannacone M, Garcia-Sastre A, Goud B, Dalod M, Moris A, Merad M, Palucka AK, Manel N (2017) Constitutive resistance to viral infection in human CD141(+) dendritic cells. Sci Immunol 2(13). https://doi.org/10.1126/sciimmunol.aai8071
Kirkling ME, Cytlak U, Lau CM, Lewis KL, Resteu A, Khodadadi-Jamayran A, Siebel CW, Salmon H, Merad M, Tsirigos A, Collin M, Bigley V, Reizis B (2018) Notch signaling facilitates in vitro generation of cross-presenting classical dendritic cells. Cell Rep 23(12):3658–3672 e3656. https://doi.org/10.1016/j.celrep.2018.05.068
doi: 10.1016/j.celrep.2018.05.068
pubmed: 29925006
pmcid: 6063084
van Eck van der Sluijs J, van Ens D, Thordardottir S, Vodegel D, Hermens I, van der Waart AB, Falkenburg JHF, Kester MGD, de Rink I, Heemskerk MHM, Borst J, Schaap NPM, Jansen JH, Xiao Y, Dolstra H, Hobo W (2021) Clinically applicable CD34(+)-derived blood dendritic cell subsets exhibit key subset-specific features and potently boost anti-tumor T and NK cell responses. Cancer Immunol Immunother 70:3167. https://doi.org/10.1007/s00262-021-02899-3
doi: 10.1007/s00262-021-02899-3