Comparison of methanol fixation versus cryopreservation of the placenta for metabolomics analysis.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 03 2023
11 03 2023
Historique:
received:
13
05
2022
accepted:
09
03
2023
entrez:
11
3
2023
pubmed:
13
3
2023
medline:
15
3
2023
Statut:
epublish
Résumé
Methods for collection of placental tissue at room temperature for metabolic profiling are described. Specimens were excised from the maternal side of the placenta and immediately flash frozen or fixed and stored for 1, 6, 12, 24, or 48 h in 80% methanol. Untargeted metabolic profiling was performed on both the methanol-fixed tissue and the methanol extract. Data were analyzed using Gaussian generalized estimating equations, two sample t-tests with false discovery rate (FDR) corrections, and principal components analysis. Methanol-fixed tissue samples and methanol extracts had a similar number of metabolites (p = 0.45, p = 0.21 in positive vs. negative ion mode). In positive ion mode, when compared to flash frozen tissue, both the methanol extract and methanol-fixed tissue (6 h) had a higher number of metabolites detected (146 additional metabolites, p
Identifiants
pubmed: 36906704
doi: 10.1038/s41598-023-31287-3
pii: 10.1038/s41598-023-31287-3
pmc: PMC10008642
doi:
Substances chimiques
Methanol
Y4S76JWI15
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4063Subventions
Organisme : NIH HHS
ID : U2C OD023375
Pays : United States
Organisme : NIH HHS
ID : U24 OD023382
Pays : United States
Organisme : NIH HHS
ID : U24 OD023319
Pays : United States
Organisme : NIH HHS
ID : UH3 OD023285
Pays : United States
Informations de copyright
© 2023. The Author(s).
Références
Sci Rep. 2016 Aug 31;6:32272
pubmed: 27578275
Metabolites. 2017 Dec 22;8(1):
pubmed: 29271872
J Dev Orig Health Dis. 2021 Apr;12(2):193-202
pubmed: 33308364
J Dev Orig Health Dis. 2021 Oct;12(5):738-747
pubmed: 33185172
BMC Clin Pathol. 2011 Dec 21;11:14
pubmed: 22188997
Sci Rep. 2018 Jan 12;8(1):663
pubmed: 29330539
Placenta. 2003 Feb-Mar;24(2-3):227-35
pubmed: 12566250
Bioinformatics. 2020 Mar 1;36(6):1801-1806
pubmed: 31642507
Ann Oncol. 2016 Mar;27(3):532-9
pubmed: 26681675
Placenta. 2013 Oct;34(10):841-5
pubmed: 23916422
Nat Prod Commun. 2009 Mar;4(3):447-54
pubmed: 19413130
Metabolites. 2018 Jan 23;8(1):
pubmed: 29360753
Nat Methods. 2020 Dec;17(12):1173-1174
pubmed: 33199890
Epigenetics. 2019 Nov;14(11):1102-1111
pubmed: 31216936
Exp Mol Pathol. 2006 Apr;80(2):183-91
pubmed: 16332367