Resveratrol attenuates staphylococcal enterotoxin B-activated immune cell metabolism
ARDS (acute respiratory disease syndrome)
MiR-100
ScRNA
T-cell metabolism
mTOR
metabolome
resveratrol
staphylococcal enterotoxin B (SEB)
Journal
Frontiers in pharmacology
ISSN: 1663-9812
Titre abrégé: Front Pharmacol
Pays: Switzerland
ID NLM: 101548923
Informations de publication
Date de publication:
2023
2023
Historique:
received:
24
11
2022
accepted:
16
02
2023
entrez:
13
3
2023
pubmed:
14
3
2023
medline:
14
3
2023
Statut:
epublish
Résumé
Acute Respiratory Distress Syndrome (ARDS) is triggered by a variety of insults, such as bacterial and viral infections, including SARS-CoV-2, leading to high mortality. In the murine model of ARDS induced by Staphylococcal enterotoxin-B (SEB), our previous studies showed that while SEB triggered 100% mortality, treatment with Resveratrol (RES) completely prevented such mortality by attenuating inflammation in the lungs. In the current study, we investigated the metabolic profile of SEB-activated immune cells in the lungs following treatment with RES. RES-treated mice had higher expression of miR-100 in the lung mononuclear cells (MNCs), which targeted mTOR, leading to its decreased expression. Also, Single-cell RNA-seq (scRNA seq) unveiled the decreased expression of mTOR in a variety of immune cells in the lungs. There was also an increase in glycolytic and mitochondrial respiration in the cells from SEB + VEH group in comparison with SEB + RES group. Together these data suggested that RES alters the metabolic reprogramming of SEB-activated immune cells, through suppression of mTOR activation and its down- and upstream effects on energy metabolism. Also, miR-100 could serve as novel potential therapeutic molecule in the amelioration of ARDS.
Identifiants
pubmed: 36909201
doi: 10.3389/fphar.2023.1106733
pii: 1106733
pmc: PMC9999031
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1106733Subventions
Organisme : NIGMS NIH HHS
ID : K99 GM147910
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI123947
Pays : United States
Informations de copyright
Copyright © 2023 Alghetaa, Mohammed, Singh, Wilson, Cai, Putluri, Nagarkatti and Nagarkatti.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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