Intestinal microbiota changes pre- and post-fecal microbiota transplantation for treatment of recurrent
Clostridioides difficile infection
fecal microbiota transplantation
inflammatory bowel disease
intestinal microbiota
recurrent CDI
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2023
2023
Historique:
received:
19
01
2023
accepted:
08
02
2023
entrez:
13
3
2023
pubmed:
14
3
2023
medline:
14
3
2023
Statut:
epublish
Résumé
Patients with inflammatory bowel disease (IBD) are at a greater risk for the recurrence of A total of 21 fecal samples were collected including 14 samples pre- and post-FMT and 7 samples from healthy donors. Microbial analysis was performed by quantitative real-time PCR (RT-qPCR) assay targeting the 16S rRNA gene. The pre-FMT profile and composition of the fecal microbiota were compared to the microbial changes of samples collected 28 days after FMT. Overall, the fecal microbiota profile of recipients was more similar to donor samples after the transplantation. We observed a significant increase in the relative abundance of Bacteroidetes post-FMT, compared to the pre-FMT microbial profile. Furthermore, there were remarkable differences between the microbial profile of pre-FMT, post-FMT, and healthy donor samples by PCoA analysis based on the ordination distance. This study demonstrates FMT as a safe and effective approach to restore the indigenous composition of the intestinal microbiota in rCDI patients and ultimately results in the treatment of concurrent IBD.
Identifiants
pubmed: 36910213
doi: 10.3389/fmicb.2023.1147945
pmc: PMC9998922
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1147945Informations de copyright
Copyright © 2023 Gholam-Mostafaei, Azimirad, Naseri, Nabavi-Rad, Asadzadeh Aghdaei, Shahrokh, Ebrahimi Daryani, Yadegar and Zali.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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