An integrated platform for high-throughput nanoscopy.
Journal
Nature biotechnology
ISSN: 1546-1696
Titre abrégé: Nat Biotechnol
Pays: United States
ID NLM: 9604648
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
02
06
2022
accepted:
02
02
2023
pmc-release:
13
09
2024
medline:
13
11
2023
pubmed:
15
3
2023
entrez:
14
3
2023
Statut:
ppublish
Résumé
Single-molecule localization microscopy enables three-dimensional fluorescence imaging at tens-of-nanometer resolution, but requires many camera frames to reconstruct a super-resolved image. This limits the typical throughput to tens of cells per day. While frame rates can now be increased by over an order of magnitude, the large data volumes become limiting in existing workflows. Here we present an integrated acquisition and analysis platform leveraging microscopy-specific data compression, distributed storage and distributed analysis to enable an acquisition and analysis throughput of 10,000 cells per day. The platform facilitates graphically reconfigurable analyses to be automatically initiated from the microscope during acquisition and remotely executed, and can even feed back and queue new acquisition tasks on the microscope. We demonstrate the utility of this framework by imaging hundreds of cells per well in multi-well sample formats. Our platform, implemented within the PYthon-Microscopy Environment (PYME), is easily configurable to control custom microscopes, and includes a plugin framework for user-defined extensions.
Identifiants
pubmed: 36914886
doi: 10.1038/s41587-023-01702-1
pii: 10.1038/s41587-023-01702-1
pmc: PMC10497732
mid: NIHMS1890782
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1549-1556Subventions
Organisme : NCI NIH HHS
ID : U01 CA200147
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA047734
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008283
Pays : United States
Organisme : NIGMS NIH HHS
ID : DP2 GM137414
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG011245
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK045735
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS128358
Pays : United States
Organisme : NIGMS NIH HHS
ID : R25 GM103792
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM118486
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008283
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA047734
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA047734
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA047734
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA047734
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM118486
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA047734
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA047734
Pays : United States
Organisme : NIGMS NIH HHS
ID : DP2 GM137414
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG011245
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK045735
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA047734
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.
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