Using pressure-driven flow systems to evaluate laser speckle contrast imaging.
dynamic light scattering
flow measurement
flow system
laser speckle contrast imaging
microfluidic
multi-exposure speckle imaging
Journal
Journal of biomedical optics
ISSN: 1560-2281
Titre abrégé: J Biomed Opt
Pays: United States
ID NLM: 9605853
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
received:
13
09
2022
accepted:
02
02
2023
entrez:
14
3
2023
pubmed:
15
3
2023
medline:
16
3
2023
Statut:
ppublish
Résumé
Microfluidic flow phantom studies are commonly used for characterizing the performance of laser speckle contrast imaging (LSCI) instruments. The selection of the flow control system is critical for the reliable generation of flow during testing. The majority of recent LSCI studies using microfluidics used syringe pumps for flow control. We quantified the uncertainty in flow generation for a syringe pump and a pressure-regulated flow system. We then assessed the performance of both LSCI and multi-exposure speckle imaging (MESI) using the pressure-regulated flow system across a range of flow speeds. The syringe pump and pressure-regulated flow systems were evaluated during stepped flow profile experiments in a microfluidic device using an inline flow sensor. The uncertainty associated with each flow system was calculated and used to determine the reliability for instrument testing. The pressure-regulated flow system was then used to characterize the relative performance of LSCI and MESI during stepped flow profile experiments while using the inline flow sensor as reference. The pressure-regulated flow system produced much more stable and reproducible flow outputs compared to the syringe pump. The expanded uncertainty for the syringe pump was 8 to Pressure-regulated flow systems should be used instead of syringe pumps when assessing the performance of flow measurement techniques with microfluidic studies. MESI offers more accurate relative flow measurements than traditional LSCI across a wide range of flow speeds.
Identifiants
pubmed: 36915371
doi: 10.1117/1.JBO.28.3.036003
pii: 220216GR
pmc: PMC10007838
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
036003Subventions
Organisme : NIBIB NIH HHS
ID : T32 EB007507
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS108484
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB011556
Pays : United States
Informations de copyright
© 2023 The Authors.
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