Impact of wavelength and spot size on laser depth of focus: Considerations for mass spectrometry imaging of non-flat samples.
depth of focus
laser wavelength
mass spectrometry imaging
spatial resolution
surface roughness
Journal
Journal of mass spectrometry : JMS
ISSN: 1096-9888
Titre abrégé: J Mass Spectrom
Pays: England
ID NLM: 9504818
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
revised:
09
02
2023
received:
04
01
2023
accepted:
19
02
2023
medline:
15
3
2023
pubmed:
15
3
2023
entrez:
14
3
2023
Statut:
ppublish
Résumé
Biospecimens with nearly flat surfaces on a flat stage are typically required for laser-based mass spectrometry imaging (MSI) techniques. However, sampling stages are rarely perfectly level, and accounting for this and the need to accommodate non-flat samples requires a deeper understanding of the laser beam depth of focus. In ablation-based MSI methods, a laser is focused on top of the sample surface, ensuring that the sample is at the focal point or remains within depth of focus. In general, the depth of focus of a given laser is related to the beam quality (M
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4914Subventions
Organisme : NIH HHS
ID : R01GM087964
Pays : United States
Informations de copyright
© 2023 The Authors. Journal of Mass Spectrometry published by John Wiley & Sons Ltd.
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