Thyme essential oil inhibits intracellular replication of CyHV-3 and inactivates extracellular virus. An in vitro study.
antiviral activity
cyprinid herpesvirus 3 (CyHV-3)
thyme essential oil (TEO)
virucidal activity
Journal
Journal of fish diseases
ISSN: 1365-2761
Titre abrégé: J Fish Dis
Pays: England
ID NLM: 9881188
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
revised:
20
02
2023
received:
30
09
2022
accepted:
23
02
2023
medline:
8
5
2023
pubmed:
15
3
2023
entrez:
14
3
2023
Statut:
ppublish
Résumé
Cyprinid herpesvirus 3 (CyHV-3) can induce up to 100% mortality among carp populations. To date, there has been no safe method to prevent the consequences of the activity of CyHV-3. Thyme is widely used in cooking due to its flavour. Both thyme and thyme essential oil (TEO) are used in traditional herbal medicine, mainly to treat respiratory system disorders. In this study, TEO containing predominantly cymene and thymol was applied to explore its antiviral effect. The toxicity of TEO was examined in MTT and crystal violet assays. The anti-CyHV-3 activity of TEO in the intracellular and extracellular stages of the viral replication cycle was explored in a plaque assay and TaqMan qPCR. TEO interfered with the intracellular stages of the CyHV-3 replication cycle with selectivity indexes (SI) of around 5. It also displayed virucidal activity in a dose- and time-dependent manner. Two-hour preincubation of CyHV-3 with TEO generated SI, ranging from 13.37 to 18.47 depending on cell line and method of examination. Preincubation of cells with TEO at a safe concentration did not decrease the intracellular viral DNA copy number, which suggests that TEO does not disturb the attachment of the virus to the cells. Further research regarding the antiviral activity of compounds of TEO is required in order to indicate the most potent molecules that could be considered candidates for application in aquaculture.
Substances chimiques
Oils, Volatile
0
thyme oil
2UK410MY6B
Antiviral Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
663-677Subventions
Organisme : Institute of Agricultural and Food Biotechnology
Organisme : Polish Ministry of Sciences and Higher Education
Organisme : Polska Akademia Nauk
Informations de copyright
© 2023 John Wiley & Sons Ltd.
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