Distinct platelet crosstalk with adaptive and innate immune cells after adenoviral and mRNA vaccination against SARS-CoV-2.


Journal

Journal of thrombosis and haemostasis : JTH
ISSN: 1538-7836
Titre abrégé: J Thromb Haemost
Pays: England
ID NLM: 101170508

Informations de publication

Date de publication:
06 2023
Historique:
received: 11 12 2022
revised: 28 02 2023
accepted: 01 03 2023
medline: 15 5 2023
pubmed: 15 3 2023
entrez: 14 3 2023
Statut: ppublish

Résumé

Genetic-based COVID-19 vaccines have proved to be highly effective in reducing the risk of hospitalization and death. Because they were first distributed in a large-scale population, the adenoviral-based vaccines were linked to a very rare thrombosis with thrombocytopenia syndrome, and the interplay between platelets and vaccinations increasingly gained attention. The objective of this article was to study the crosstalk between platelets and the vaccine-induced immune response. We prospectively enrolled young healthy volunteers who received the mRNA-based vaccine, BNT162b2 (n = 15), or the adenovirus-based vaccine, AZD1222 (n = 25) and studied their short-term platelet and immune response before and after vaccine injections. In a separate cohort, we retrospectively analyzed the effect of aspirin on the antibody response 1 and 5 months after BNT162b2 vaccination. Here, we show that a faster antibody response to either vaccine is associated with the formation of platelet aggregates with marginal zone-like B cells, a subset geared to bridge the temporal gap between innate and adaptive immunities. However, although the mRNA-based vaccine is associated with a more gradual and tolerogenic response that fosters the crosstalk between platelets and adaptive immunity, the adenovirus-based vaccine, the less immunogenic of the 2, evokes an antiviral-like response during which the platelets are cleared and less likely to cooperate with B cells. Moreover, subjects taking aspirin (n = 56) display lower antibody levels after BNT162b2 vaccination compared with matched individuals. Platelets are a component of the innate immune pathways that promote the B-cell response after vaccination. Future studies on the platelet-immune crosstalk post-immunization will improve the safety, efficacy, and strategic administration of next-generation vaccines.

Sections du résumé

BACKGROUND
Genetic-based COVID-19 vaccines have proved to be highly effective in reducing the risk of hospitalization and death. Because they were first distributed in a large-scale population, the adenoviral-based vaccines were linked to a very rare thrombosis with thrombocytopenia syndrome, and the interplay between platelets and vaccinations increasingly gained attention.
OBJECTIVES
The objective of this article was to study the crosstalk between platelets and the vaccine-induced immune response.
METHODS
We prospectively enrolled young healthy volunteers who received the mRNA-based vaccine, BNT162b2 (n = 15), or the adenovirus-based vaccine, AZD1222 (n = 25) and studied their short-term platelet and immune response before and after vaccine injections. In a separate cohort, we retrospectively analyzed the effect of aspirin on the antibody response 1 and 5 months after BNT162b2 vaccination.
RESULTS
Here, we show that a faster antibody response to either vaccine is associated with the formation of platelet aggregates with marginal zone-like B cells, a subset geared to bridge the temporal gap between innate and adaptive immunities. However, although the mRNA-based vaccine is associated with a more gradual and tolerogenic response that fosters the crosstalk between platelets and adaptive immunity, the adenovirus-based vaccine, the less immunogenic of the 2, evokes an antiviral-like response during which the platelets are cleared and less likely to cooperate with B cells. Moreover, subjects taking aspirin (n = 56) display lower antibody levels after BNT162b2 vaccination compared with matched individuals.
CONCLUSION
Platelets are a component of the innate immune pathways that promote the B-cell response after vaccination. Future studies on the platelet-immune crosstalk post-immunization will improve the safety, efficacy, and strategic administration of next-generation vaccines.

Identifiants

pubmed: 36918114
pii: S1538-7836(23)00229-5
doi: 10.1016/j.jtha.2023.03.003
pmc: PMC10008173
pii:
doi:

Substances chimiques

BNT162 Vaccine 0
COVID-19 Vaccines 0
ChAdOx1 nCoV-19 B5S3K2V0G8
Aspirin R16CO5Y76E

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1636-1649

Informations de copyright

Copyright © 2023 International Society on Thrombosis and Haemostasis. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interests The authors declare no conflicts of interest.

Auteurs

Ludovica Lombardi (L)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Francesca Maiorca (F)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Ramona Marrapodi (R)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Annamaria Sabetta (A)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Noemi Scafa (N)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Davide Pallucci (D)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Marzia Miglionico (M)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Giulio Francesco Romiti (GF)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Bernadette Corica (B)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Silvia Piconese (S)

Department of Internal Clinical Sciences, Anesthesiology and Cardiovascular Sciences, Sapienza University of Rome, Rome, Italy.

Antonella Polimeni (A)

Department of Oral and Maxillo-Facial Sciences, Sapienza University of Rome, Rome, Italy.

Fabio Pulcinelli (F)

Department of Experimental Medicine, Sapienza University, Rome, Italy.

Roberto Cangemi (R)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Marcella Visentini (M)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Stefania Basili (S)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.

Lucia Stefanini (L)

Department of Translational and Precision Medicine, Sapienza University, Rome, Italy; Istituto Pasteur Italia - Fondazione Cenci Bolognetti, Rome, Italy. Electronic address: lucia.stefanini@uniroma1.it.

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Classifications MeSH