The Anti-Helicobacter pylori effects of Limosilactobacillus reuteri strain 2892 isolated from Camel milk in C57BL/6 mice.


Journal

World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472

Informations de publication

Date de publication:
15 Mar 2023
Historique:
received: 01 10 2022
accepted: 24 02 2023
entrez: 15 3 2023
pubmed: 16 3 2023
medline: 17 3 2023
Statut: epublish

Résumé

Helicobacter pylori infection (H. pylori) is associated with chronic gastritis, peptic ulcers, and gastric cancer. The present study provides information on the protective effects of Limosilactobacillus reuteri strain 2892 (L. reuteri 2892) isolated from camel's milk against H. pylori-induced gastritis in the stomach tissue of animal models. Animal assays revealed that L. reuteri 2892 pretreatment significantly downregulated the virulence factor cagA gene expression. It upregulated the expression level of tight junction molecules [zona occludens (ZO-1), claudin-4] and suppressed metalloproteinase (MMP)-2 and MMP-9 expressions. L. reuteri 2892 exhibited immunomodulatory effects on cytokine profile, as it reduced the serum concentrations of pro-inflammatory cytokines interleukin (IL)-6, IL-1β, and INF-γ and increased the anti-inflammatory cytokine, IL-10. In addition, L. reuteri 2892 showed anti-oxidative stress activity by regulating the levels of oxidative stress-associated markers [superoxide dismutase (SOD) and malondialdehyde (MDA)]. Our findings suggest that L. reuteri 2892 attenuates H. pylori-induced gastritis.

Identifiants

pubmed: 36918449
doi: 10.1007/s11274-023-03555-x
pii: 10.1007/s11274-023-03555-x
doi:

Substances chimiques

Cytokines 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

119

Subventions

Organisme : Islamic Azad University of Shiraz
ID : 162449566

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer Nature B.V.

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Auteurs

Fatemeh Forooghi Nia (F)

Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.

Atefe Rahmati (A)

Department of Hematology and Blood Banking, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Basic Sciences, Faculty of Medicine, Neyshabur University of Medical Sciences, Neyshabur, Iran.

Mona Ariamanesh (M)

Department of Pathology, Faculty of Medicine, Neyshabur University of Medical Sciences, Neyshabur, Iran.

Jafar Saeidi (J)

Department of Physiology, School of Basic Science, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.

Ahmad Ghasemi (A)

Department of Basic Sciences, Faculty of Medicine, Neyshabur University of Medical Sciences, Neyshabur, Iran. Ghasemia2@nums.ac.ir.
Healthy Ageing Research Centre, Neyshabur University of Medical Sciences, Neyshabur, Iran. Ghasemia2@nums.ac.ir.

Mahnaz Mohtashami (M)

Department of Microbiology, School of Basic Science, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran. Mahnazmohtashami@gmail.com.

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