MicroRNA Profiling of Self-Renewing Human Neural Stem Cells Reveals Novel Sets of Differentially Expressed microRNAs During Neural Differentiation In Vitro.
Cell cycle
Human pluripotent stem cells
Neural stem cells
miRNA sequencing
microRNA
Journal
Stem cell reviews and reports
ISSN: 2629-3277
Titre abrégé: Stem Cell Rev Rep
Pays: United States
ID NLM: 101752767
Informations de publication
Date de publication:
07 2023
07 2023
Historique:
accepted:
28
02
2023
medline:
27
7
2023
pubmed:
16
3
2023
entrez:
15
3
2023
Statut:
ppublish
Résumé
The involvement of microRNAs (miRNAs) in orchestrating self-renewal and differentiation of stem cells has been revealed in a number of recent studies. And while in human pluripotent stem cells, miRNAs have been directly linked to the core pluripotency network, including the cell cycle regulation and the maintenance of the self-renewing capacity, their role in the onset of differentiation in other contexts, such as determination of neural cell fate, remains poorly described. To bridge this gap, we used three model cell types to study miRNA expression patterns: human embryonic stem cells (hESCs), hESCs-derived self-renewing neural stem cells (NSCs), and differentiating NSCs. The comprehensive miRNA profiling presented here reveals novel sets of miRNAs differentially expressed during human neural cell fate determination in vitro. Furthermore, we report a miRNA expression profile of self-renewing human NSCs, which has been lacking to this date. Our data also indicates that miRNA clusters enriched in NSCs share the target-determining seed sequence with cell cycle regulatory miRNAs expressed in pluripotent hESCs. Lastly, our mechanistic experiments confirmed that cluster miR-17-92, one of the NSCs-enriched clusters, is directly transcriptionally regulated by transcription factor c-MYC.
Identifiants
pubmed: 36918496
doi: 10.1007/s12015-023-10524-2
pii: 10.1007/s12015-023-10524-2
pmc: PMC10366325
doi:
Substances chimiques
MicroRNAs
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1524-1539Informations de copyright
© 2023. The Author(s).
Références
Stem Cells. 2012 Jul;30(7):1362-72
pubmed: 22511267
Oncogene. 2009 Jan 8;28(1):20-30
pubmed: 18806832
Nucleic Acids Res. 2010 Nov;38(20):6895-905
pubmed: 20624818
Cell Tissue Res. 2018 Jan;371(1):47-54
pubmed: 28831605
Cold Spring Harb Perspect Med. 2014 Jun 02;4(6):
pubmed: 24890832
Mol Cell Biol. 2008 Nov;28(21):6609-19
pubmed: 18725401
Genes Dev. 2007 Apr 1;21(7):744-9
pubmed: 17403776
Oncogene. 2022 Nov;41(45):4960-4970
pubmed: 36207533
Cancer Cell. 2014 Aug 11;26(2):262-72
pubmed: 25117713
Nucleic Acids Res. 2019 Jan 8;47(D1):D155-D162
pubmed: 30423142
Genes Dev. 2006 Jun 1;20(11):1511-24
pubmed: 16705040
Development. 2008 Dec;135(23):3911-21
pubmed: 18997113
PLoS One. 2013;8(3):e59011
pubmed: 23527072
Proc Natl Acad Sci U S A. 2005 Nov 8;102(45):16426-31
pubmed: 16260724
Cell Rep. 2013 May 30;3(5):1398-1406
pubmed: 23623502
Genome Biol. 2009;10(3):R25
pubmed: 19261174
Nat Protoc. 2006;1(1):179-85
pubmed: 17406230
J Cell Sci. 2003 Dec 15;116(Pt 24):4947-55
pubmed: 14625388
PLoS One. 2012;7(5):e38174
pubmed: 22693596
FASEB J. 2019 Apr;33(4):5257-5267
pubmed: 30668139
Mol Cell Biol. 2013 May;33(9):1782-96
pubmed: 23438603
Science. 1998 Nov 6;282(5391):1145-7
pubmed: 9804556
Nucleic Acids Res. 2019 Apr 23;47(7):3353-3364
pubmed: 30820533
J Exp Clin Cancer Res. 2018 Jul 27;37(1):173
pubmed: 30053872
Mol Cancer. 2021 Jan 4;20(1):3
pubmed: 33397405
Trends Cell Biol. 2010 May;20(5):233-43
pubmed: 20153966
Neural Dev. 2013 Jul 29;8:14
pubmed: 23895693
Genome Biol. 2014;15(12):550
pubmed: 25516281
Nat Cell Biol. 2019 Sep;21(9):1060-1067
pubmed: 31481793
J Cell Physiol. 2006 Dec;209(3):883-93
pubmed: 16972248
Nat Struct Mol Biol. 2009 Apr;16(4):365-71
pubmed: 19330006
Results Probl Cell Differ. 2011;53:459-72
pubmed: 21630156
Stem Cells Dev. 2017 Nov 1;26(21):1521-1527
pubmed: 28835165
Cell Rep. 2022 Dec 6;41(10):111773
pubmed: 36476846
PLoS One. 2010 Oct 18;5(10):e13453
pubmed: 20976144
Int J Mol Sci. 2019 Jul 26;20(15):
pubmed: 31357387
J Cell Physiol. 2010 Jan;222(1):103-10
pubmed: 19774559
Nucleic Acids Res. 2010 Mar;38(5):e34
pubmed: 20008100
Int J Mol Sci. 2020 Sep 23;21(19):
pubmed: 32977388
Nucleic Acids Res. 2005 May 12;33(8):2697-706
pubmed: 15891114
Nat Neurosci. 2009 Apr;12(4):399-408
pubmed: 19287386
Stem Cells. 2012 Jun;30(6):1097-108
pubmed: 22415928
Development. 2020 May 11;147(9):
pubmed: 32273274
Genomics. 2007 Jun;89(6):687-96
pubmed: 17418524
Neuronal Signal. 2018 Jan 26;2(1):NS20170181
pubmed: 32714583
Proc Natl Acad Sci U S A. 2010 Nov 23;107(47):20382-7
pubmed: 21059906
Nature. 2005 Jun 9;435(7043):839-43
pubmed: 15944709
Nat Commun. 2022 Jun 27;13(1):3696
pubmed: 35760790
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
Aging (Albany NY). 2011 Feb;3(2):108-24
pubmed: 21386132
J Neurosci. 2005 Jul 13;25(28):6533-8
pubmed: 16014714
Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):1876-81
pubmed: 20133835
Bioinformatics. 2011 Nov 15;27(22):3202-3
pubmed: 21976421
Methods. 2013 Sep 1;63(1):41-9
pubmed: 23816787
Endocrinology. 2004 Dec;145(12):5439-47
pubmed: 15331580
Cell Stem Cell. 2009 Sep 4;5(3):320-31
pubmed: 19733543
Biochem Biophys Res Commun. 2009 Dec 4;390(1):1-4
pubmed: 19769940
Nat Commun. 2011 Nov 08;2:529
pubmed: 22068596
Stem Cells Dev. 2013 Jan 15;22(2):279-95
pubmed: 22827698
Cell Tissue Res. 2015 Jan;359(1):47-64
pubmed: 25172833
Antioxidants (Basel). 2021 Sep 27;10(10):
pubmed: 34679668
Stem Cell Res. 2007 Oct;1(1):45-60
pubmed: 19383386
Dev Biol. 2004 Jun 15;270(2):488-98
pubmed: 15183728
Nat Commun. 2016 Mar 11;7:10965
pubmed: 26965827
Stem Cell Res Ther. 2019 Mar 12;10(1):83
pubmed: 30867054
Stem Cell Res. 2013 Jan;10(1):118-31
pubmed: 23178806
Cell Mol Life Sci. 2014 Aug;71(16):2975-95
pubmed: 24519472
RNA Biol. 2019 Jan;16(1):69-81
pubmed: 30582411
Regen Ther. 2022 May 31;21:37-45
pubmed: 35702483
Cell. 2013 Sep 26;155(1):135-47
pubmed: 24074866
Mol Cell Biol. 2005 Nov;25(21):9198-208
pubmed: 16227573
Epilepsia. 2017 Oct;58(10):1782-1793
pubmed: 28815576
Curr Opin Neurobiol. 2008 Feb;18(1):108-15
pubmed: 18514504
Cell Cycle. 2010 May 15;9(10):1893-900
pubmed: 20436288
Nucleic Acids Res. 2016 Jan 4;44(D1):D717-25
pubmed: 26590259
J Neurosci. 2013 Apr 17;33(16):6885-94
pubmed: 23595747
Nat Genet. 2003 Nov;35(3):215-7
pubmed: 14528307
Leukemia. 2019 Feb;33(2):403-414
pubmed: 30111844
Stem Cell Res. 2021 May;53:102378
pubmed: 34088007
Nat Commun. 2012;3:1244
pubmed: 23212373
Mol Biol Evol. 2016 Sep;33(9):2232-47
pubmed: 27189568
Cell. 2008 Aug 8;134(3):521-33
pubmed: 18692474
Stem Cell Res. 2021 May;53:102379
pubmed: 34088008
Mol Cell Biol. 2008 Oct;28(20):6426-38
pubmed: 18710938