An insight into gut microbiota and metabolites in the mice with adenomyosis.
adenomyosis
fecal metabolites
glutathione
gut microbiota
sex hormone
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2023
2023
Historique:
received:
20
10
2022
accepted:
07
02
2023
entrez:
16
3
2023
pubmed:
17
3
2023
medline:
21
3
2023
Statut:
epublish
Résumé
Adenomyosis (AM) is a benign uterine disease characterized pathologically by the invasion of endometrial tissue into the myometrium. The pathogenesis of AM is still far from clear. Although the gut microbiome and metabolomics are thought to contribute to a variety of diseases, the role of them in AM has not been revealed. To investigate changes in the gut microbiota and derived metabolites in AM mice. Female ICR mice were randomly assigned to AM and control groups, and pituitary transplantation was employed to perform AM modeling. Then, the fecal samples were obtained for microbial (16S rRNA gene sequencing) and metabolomic (liquid chromatography mass spectrometry, LC-MS) analysis. The results of gut microbiota analysis showed that the intestinal microbiota composition of AM mice was altered. The ratio of AM mice have a unique gut microbiome and intestinal metabolites.
Sections du résumé
Background
Adenomyosis (AM) is a benign uterine disease characterized pathologically by the invasion of endometrial tissue into the myometrium. The pathogenesis of AM is still far from clear. Although the gut microbiome and metabolomics are thought to contribute to a variety of diseases, the role of them in AM has not been revealed.
Objective
To investigate changes in the gut microbiota and derived metabolites in AM mice.
Method
Female ICR mice were randomly assigned to AM and control groups, and pituitary transplantation was employed to perform AM modeling. Then, the fecal samples were obtained for microbial (16S rRNA gene sequencing) and metabolomic (liquid chromatography mass spectrometry, LC-MS) analysis.
Result
The results of gut microbiota analysis showed that the intestinal microbiota composition of AM mice was altered. The ratio of
Conclusion
AM mice have a unique gut microbiome and intestinal metabolites.
Identifiants
pubmed: 36923594
doi: 10.3389/fcimb.2023.1075387
pmc: PMC10008959
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1075387Informations de copyright
Copyright © 2023 Chen, Wang, Zhuang, Fu, Liu, Chen and Lei.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Sci Rep. 2020 Jul 23;10(1):12283
pubmed: 32704056
J Cell Biol. 2016 Aug 1;214(3):249-57
pubmed: 27458133
World J Gastroenterol. 2015 Aug 7;21(29):8787-803
pubmed: 26269668
Gut Microbes. 2021 Jan-Dec;13(1):1-21
pubmed: 33722164
Obes Rev. 2018 Apr;19(4):557-575
pubmed: 29356299
Appl Environ Microbiol. 2020 Oct 28;86(22):
pubmed: 32859601
Microorganisms. 2020 Nov 01;8(11):
pubmed: 33139627
Cancer Epidemiol Biomarkers Prev. 2021 Nov;30(11):2018-2029
pubmed: 34446472
J Assist Reprod Genet. 2022 May;39(5):1027-1031
pubmed: 35347501
Cancer Res. 2021 Feb 1;81(3):552-566
pubmed: 33229341
J Clin Med. 2021 Oct 22;10(21):
pubmed: 34768397
Clin Lab. 2022 Sep 1;68(9):
pubmed: 36125161
Mass Spectrom Rev. 2017 May;36(3):450-470
pubmed: 26633258
Cells. 2020 Mar 12;9(3):
pubmed: 32178484
Obes Res Clin Pract. 2022 May-Jun;16(3):235-241
pubmed: 35431154
Front Immunol. 2022 May 11;13:874021
pubmed: 35634334
Aust Fam Physician. 2017;46(4):206-211
pubmed: 28376573
Am J Reprod Immunol. 2020 Dec;84(6):e13307
pubmed: 32681566
Front Endocrinol (Lausanne). 2020 Sep 08;11:628
pubmed: 33013704
Front Cell Infect Microbiol. 2022 Aug 12;12:968992
pubmed: 36034713
Biomedicines. 2022 Jan 14;10(1):
pubmed: 35052854
Fertil Steril. 2013 Sep;100(3):801-9
pubmed: 23755957
EMBO J. 2017 May 15;36(10):1302-1315
pubmed: 28420743
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6
pubmed: 23193283
J Cell Biol. 2018 Jul 2;217(7):2291-2298
pubmed: 29915025
Evid Based Complement Alternat Med. 2020 Jul 20;2020:3687498
pubmed: 32765629
Fertil Steril. 2022 Jun;117(6):1235-1245
pubmed: 35367064
J Clin Med. 2021 Mar 05;10(5):
pubmed: 33807739
Semin Reprod Med. 2020 May;38(2-03):168-178
pubmed: 33105508
Sci Rep. 2019 Feb 18;9(1):2204
pubmed: 30778155
Arch Gynecol Obstet. 2021 Nov;304(5):1363-1373
pubmed: 33839907
Reprod Sci. 2021 Aug;28(8):2367-2377
pubmed: 33660232
BMC Microbiol. 2021 Dec 11;21(1):339
pubmed: 34895145
Reprod Biol Endocrinol. 2022 Mar 9;20(1):49
pubmed: 35264202
Antonie Van Leeuwenhoek. 2020 Dec;113(12):2019-2040
pubmed: 33136284
Cells. 2022 Feb 13;11(4):
pubmed: 35203298
Fertil Steril. 2021 Jul;116(1):243-254
pubmed: 33849709
Nutrients. 2017 Jul 25;9(8):
pubmed: 28757576
Acta Obstet Gynecol Scand. 2021 Jul;100(7):1248-1257
pubmed: 33550615
Nat Microbiol. 2022 Mar;7(3):434-450
pubmed: 35241796
Front Cell Infect Microbiol. 2021 Dec 07;11:788836
pubmed: 34950610
Sci Rep. 2020 Sep 10;10(1):14886
pubmed: 32913258
ScientificWorldJournal. 2021 Apr 20;2021:6653677
pubmed: 33986637
Med Sci Sports Exerc. 2020 Jan;52(1):94-104
pubmed: 31425383
Compr Physiol. 2018 Sep 14;8(4):1253-1290
pubmed: 30215860
Reprod Biomed Online. 2017 Nov;35(5):592-601
pubmed: 28693952