RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits.

Animals RNA Polymerase II / metabolism Transcription, Genetic Ribosomal Proteins / genetics Ribosomes / metabolism Protein Subunits / genetics Mammals / metabolism
3′ end processing RNA Pol II subunits RNA polymerase RPB3 protein degradation ribosomal protein genes transcription

Journal

Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571

Informations de publication

Date de publication:
20 04 2023
Historique:
received: 30 03 2022
revised: 12 12 2022
accepted: 23 02 2023
medline: 25 4 2023
pubmed: 17 3 2023
entrez: 16 3 2023
Statut: ppublish

Résumé

RNA polymerase II (RNA Pol II) has been recognized as a passively regulated multi-subunit holoenzyme. However, the extent to which RNA Pol II subunits might be important beyond the RNA Pol II complex remains unclear. Here, fractions containing disassociated RPB3 (dRPB3) were identified by size exclusion chromatography in various cells. Through a unique strategy, i.e., "specific degradation of disassociated subunits (SDDS)," we demonstrated that dRPB3 functions as a regulatory component of RNA Pol II to enable the preferential control of 3' end processing of ribosomal protein genes directly through its N-terminal domain. Machine learning analysis of large-scale genomic features revealed that the little elongation complex (LEC) helps to specialize the functions of dRPB3. Mechanistically, dRPB3 facilitates CBC-PCF11 axis activity to increase the efficiency of 3' end processing. Furthermore, RPB3 is dynamically regulated during development and diseases. These findings suggest that RNA Pol II gains specific regulatory functions by trapping disassociated subunits in mammalian cells.

Identifiants

DOI: 10.1016/j.molcel.2023.02.028 PMID: 36924766
pubmed: 36924766
pii: S1097-2765(23)00155-7
doi: 10.1016/j.molcel.2023.02.028
pii:
doi:

Substances chimiques

RNA Polymerase II EC 2.7.7.-
Ribosomal Proteins 0
Protein Subunits 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1280-1297.e11

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Yuanjun Li (Y)

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Jie Huang (J)

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Lijun Bao (L)

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Junyi Zhu (J)

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Wenjia Duan (W)

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Haonan Zheng (H)

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Hui Wang (H)

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Yongpeng Jiang (Y)

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Weiwei Liu (W)

Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

Meiling Zhang (M)

State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Department of Chemical Biology and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

Yang Yu (Y)

Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

Chengqi Yi (C)

State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Department of Chemical Biology and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

Xiong Ji (X)

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China. Electronic address: xiongji@pku.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › RNA Pol II preferentially regulates ribosomal...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › DNA-directed RNA polymerases › RNA polymerase II › RNA Pol II preferentially regulates ribosomal...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Transcription génétique › RNA Pol II preferentially regulates ribosomal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines ribosomiques › RNA Pol II preferentially regulates ribosomal...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Ribosomes › RNA Pol II preferentially regulates ribosomal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Sous-unités de protéines › RNA Pol II preferentially regulates ribosomal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › RNA Pol II preferentially regulates ribosomal...