Evaluation of the main regulators of systemic iron homeostasis in pyruvate kinase deficiency.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
16 03 2023
Historique:
received: 15 11 2022
accepted: 14 03 2023
entrez: 17 3 2023
pubmed: 18 3 2023
medline: 21 3 2023
Statut: epublish

Résumé

Iron homeostasis and dyserythropoiesis are poorly investigated in pyruvate kinase deficiency (PKD), the most common glycolytic defect of erythrocytes. Herein, we studied the main regulators of iron balance and erythropoiesis, as soluble transferrin receptor (sTfR), hepcidin, erythroferrone (ERFE), and erythropoietin (EPO), in a cohort of 41 PKD patients, compared with 42 affected by congenital dyserythropoietic anemia type II (CDAII) and 50 with hereditary spherocytosis (HS). PKD patients showed intermediate values of hepcidin and ERFE between CDAII and HS, and clear negative correlations between log-transformed hepcidin and log-EPO (Person's r correlation coefficient =  - 0.34), log-hepcidin and log-ERFE (r =  - 0.47), and log-hepcidin and sTfR (r =  - 0.44). sTfR was significantly higher in PKD; EPO levels were similar in PKD and CDAII, both higher than in HS. Finally, genotype-phenotype correlation in PKD showed that more severe patients, carrying non-missense/non-missense genotypes, had lower hepcidin and increased ERFE, EPO, and sTFR compared with the others (missense/missense and missense/non-missense), suggesting a higher rate of ineffective erythropoiesis. We herein investigated the main regulators of systemic iron homeostasis in the largest cohort of PKD patients described so far, opening new perspectives on the molecular basis and therapeutic approaches of this disease.

Identifiants

pubmed: 36927785
doi: 10.1038/s41598-023-31571-2
pii: 10.1038/s41598-023-31571-2
pmc: PMC10020532
doi:

Substances chimiques

Hepcidins 0
Iron E1UOL152H7
Erythropoietin 11096-26-7
Receptors, Transferrin 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4395

Informations de copyright

© 2023. The Author(s).

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Auteurs

Anna Zaninoni (A)

Hematology Unit, Pathophysiology of Anemias Unit, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.

Roberta Marra (R)

Department of Molecular Medicine and Medical Biotechnology, University Federico II, Naples, Italy.
CEINGE - Biotecnologie Avanzate Franco Salvatore, Naples, Italy.

Elisa Fermo (E)

Hematology Unit, Pathophysiology of Anemias Unit, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.

Dario Consonni (D)

Epidemiology Unit, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.

Immacolata Andolfo (I)

Department of Molecular Medicine and Medical Biotechnology, University Federico II, Naples, Italy.
CEINGE - Biotecnologie Avanzate Franco Salvatore, Naples, Italy.

Anna Paola Marcello (AP)

Hematology Unit, Pathophysiology of Anemias Unit, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.

Barbara Eleni Rosato (BE)

Department of Molecular Medicine and Medical Biotechnology, University Federico II, Naples, Italy.
CEINGE - Biotecnologie Avanzate Franco Salvatore, Naples, Italy.

Cristina Vercellati (C)

Hematology Unit, Pathophysiology of Anemias Unit, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.

Wilma Barcellini (W)

Hematology Unit, Pathophysiology of Anemias Unit, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.

Achille Iolascon (A)

Department of Molecular Medicine and Medical Biotechnology, University Federico II, Naples, Italy. achille.iolascon@unina.it.
CEINGE - Biotecnologie Avanzate Franco Salvatore, Naples, Italy. achille.iolascon@unina.it.

Paola Bianchi (P)

Hematology Unit, Pathophysiology of Anemias Unit, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. paola.bianchi@policlinico.mi.it.

Roberta Russo (R)

Department of Molecular Medicine and Medical Biotechnology, University Federico II, Naples, Italy.
CEINGE - Biotecnologie Avanzate Franco Salvatore, Naples, Italy.

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