Assessment of anti-psoriatic activity of bakuchiol-loaded solid lipid nanoparticles-based gel: design, characterization, and mechanistic insight via NF-kB signaling pathway.
Bakuchiol
Inflammation
Psoralea corylifolia
Psoriasis
Topical therapy
Journal
Naunyn-Schmiedeberg's archives of pharmacology
ISSN: 1432-1912
Titre abrégé: Naunyn Schmiedebergs Arch Pharmacol
Pays: Germany
ID NLM: 0326264
Informations de publication
Date de publication:
09 2023
09 2023
Historique:
received:
19
11
2022
accepted:
22
02
2023
medline:
9
8
2023
pubmed:
18
3
2023
entrez:
17
3
2023
Statut:
ppublish
Résumé
The aim of the current study is to evaluate the anti-psoriatic potential of bakuchiol (Bak) loaded solid lipid nanoparticles (SLNs) via modulating inflammatory and oxidative pathways. Bak-loaded SLNs were prepared using hot homogenization method and characterized by various spectroscopic techniques. Bak-SLNs suspension was formulated into gel using Carbopol. Different in vivo assays were executed to explore the role of inflammatory markers and oxidative enzymes in psoriasis. DLS (dynamic light scattering) analysis showed suitable particle size, zeta potential, and polydispersity index (PDI) of developed formulation. TEM (transmission electron microscopy) reveal the spherical shape of Bak-SLNs particles. The release studies confirmed the sustained release of Bak-SLNs-based gel. UV-B-induced psoriatic Wistar rat model showed significant anti-psoriatic effect of Bak via regulating inflammatory markers (NF-kB, IL-6, IL-4, and IL-10) and levels of anti-oxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione-S-transferase (GST). Furthermore, RT-qPCR analysis confirms that Bak downregulates the expression of inflammatory markers, while histology and immunohistology results also confirm the anti-psoriatic effect of Bak. The study indicates that Bak-loaded SLNs-based gel significantly downregulates the level of cytokines and interleukins involve in NF-kB signaling cascade; hence, it can prove to be a novel therapeutic approach to cure psoriasis.
Identifiants
pubmed: 36929274
doi: 10.1007/s00210-023-02445-1
pii: 10.1007/s00210-023-02445-1
doi:
Substances chimiques
Lipid Nanoparticles
0
NF-kappa B
0
bakuchiol
OT12HJU3AR
Glutathione
GAN16C9B8O
Drug Carriers
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Retracted Publication
Langues
eng
Sous-ensembles de citation
IM
Pagination
2105-2125Commentaires et corrections
Type : RetractionIn
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Alghasham A, Rasheed Z (2014) Therapeutic targets for rheumatoid arthritis: progress and promises. Autoimmunity 47:77–94
Anderson ME (1985) Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol 113:548–555
Azfar RS, Gelfand JM (2008) Psoriasis and metabolic disease: epidemiology and pathophysiology. Curr Opin Rheumatol 20:416
Bailey EE, Ference EH, Alikhan A (2012) Armstrong, Combination treatments for psoriasis: a systematic review and meta-analysis. Arch Dermatol 148:511–522
Baran A, Flisiak I, Jaroszewicz J (2015) Effect of psoriasis activity on serum adiponectin and leptin levels. Adv Dermatol Allergology/Postȩpy Dermatol Alergol 32:101
Battaglia L, Gallarate M (2012) Lipid nanoparticles: state of the art, new preparation methods and challenges in drug delivery. Expert Opin Drug Deliv 9:497–508
Beloqui A, Solinís MÁ, Rodríguez-Gascón A (2016) Nanostructured lipid carriers: promising drug delivery systems for future clinics. Nanomedicine: Nanotechnology. Biol Med 12:143–161
Busard C, Zweegers J, Limpens J (2014) Spuls, Combined use of systemic agents for psoriasis: a systematic review. JAMA Dermatol 150:1213–1220
Cather JC, Crowley JJ (2014) Use of biologic agents in combination with other therapies for the treatment of psoriasis. Am J Clin Dermatol 15:467–478
Coimbra S, Oliveira H, Reis F (2010) Circulating adipokine levels in Portuguese patients with psoriasis vulgaris according to body mass index, severity and therapy. J Eur Acad Dermatol Venereol 24:1386–1394
Derakhshan N (2011) NFκB inhibitors as a potential novel hypothesized treatment for psoriasis. Sao Paulo Med J 129:433–434
Di Cesare A, Di Meglio P, Nestle FO (2009) The IL-23/Th17 axis in the immunopathogenesis of psoriasis. J Investig Dermatol 129:1339–1350
Duong VA, Nguyen TTL, Maeng HJ (2020) Preparation of solid lipid nanoparticles and nanostructured lipid carriers for drug delivery and the effects of preparation parameters of solvent injection method. Molecules 25:4781
Fereig SA, El-Zaafarany GM, Arafa MG (2020) Tackling the various classes of nano-therapeutics employed in topical therapy of psoriasis. Drug Delivery 27:662–680
Frank O, Nestle M, Daniel H (2009) Mechanisms of disease: psoriasis. N Engl J Med 61:496–509
Gabriel D, Mugnier T, Courthion H (2016) Improved topical delivery of tacrolimus: a novel composite hydrogel formulation for the treatment of psoriasis. J Control Release 242:16–24
Garg T (2016) Current nanotechnological approaches for an effective delivery of bio-active drug molecules in the treatment of acne. Artif Cells Nanomed Biotechnol 44:98–105
Gisondi P, Altomare G, Ayala F (2017) Italian guidelines on the systemic treatments of moderate-to-severe plaque psoriasis. J Eur Acad Dermatol Venereol 31:774–790
Goldminz AM, Au SC, Kim N (2013) NF-κB: an essential transcription factor in psoriasis. J Dermatol Sci 69:89–94
Grinberg-Bleyer Y, Dainichi T, Oh H (2015) Cutting edge: NF-κB p65 and c-Rel control epidermal development and immune homeostasis in the skin. J Immunol 194:2472–2476
Guagnano MT, Romano M, Falco A (2003) Leptin increase is associated with markers of the hemostatic system in obese healthy women. J Thromb Haemost 1:2330–2334
Habig WH, Pabst MJ, Jakoby WBC (1974) Glutathione-S-transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139
Ho HN, Le TG, Dao TTT (2020) Development of itraconazole-loaded polymeric nanoparticle dermal gel for enhanced antifungal efficacy. J Nanomater 2020:1–11
Hunter JE, Fowler JF Jr (1998) Safety to human skin of cocamidopropyl betaine: a mild surfactant for personal-care products. J Surfactants Deterg 1:235–239
Jain AK, Jain A, Garg NK, Agarwal A, Jain A, Jain SA, Tyagi RK, Jain RK, Agrawal H, Agrawal GP (2014) Adapalene loaded solid lipid nanoparticles gel: an effective approach for acne treatment. Colloids Surf, B 121:222–229
Kakkar P, Das B, Viswanathan PN (1978) A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophy 21:130–132
Kamiya K, Kishimoto M, Sugai J (2019) Risk factors for the development of psoriasis. Int J Mol Sci 20:4347
Kaur S, Zilmer K, Leping V (2011) The levels of adiponectin and leptin and their relation to other markers of cardiovascular risk in patients with psoriasis. J Eur Acad Dermatol Venereol 25:1328–1333
Kaur A, Katiyar SS, Kushwah V (2017) Nanoemulsion loaded gel for topical co-delivery of clobitasol propionate and calcipotriol in psoriasis. Nanomedicine: Nanotechnology. Biology and Medicine 13:1473–1482
Kaur L, Singh K, Paul S (2018) A mechanistic study to determine the structural similarities between artificial membrane Strat-M™ and biological membranes and its application to carry out skin permeation study of amphotericin B nanoformulations. AAPS PharmSciTech 19:1606–1624
Khan AW, Kotta S, Ansari SH (2013) Formulation development, optimization and evaluation of aloe vera gel for wound healing. Pharmacogn Mag 9:S6
Khan MFA, Howari H, Alhodaib A (2022) Hydrogel containing solid lipid nanoparticles loaded with argan oil and simvastatin: preparation, in vitro and ex vivo assessment. Gels 8:277
Krishna TA, Edachery B, Athalathil S (2022) Bakuchiol–a natural meroterpenoid: structure, isolation, synthesis and functionalization approaches. RSC Adv 12:8815–8832
La Russa D, Marrone A, Mandalà M (2020) Antioxidant/anti-inflammatory effects of caloric restriction in an aged and obese rat Model: The role of adiponectin. Biomedicines 8:532
Lambers H, Piessens S, Bloem A (2006) Natural skin surface pH is on average below 5, which is beneficial for its resident flora. Int J Cosmet Sci 28:359–370
Lawrence T (2009) The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol 1:a001651
Lewińska A, Domżał-Kędzia M, Maciejczyk E (2021) Design and engineering of “green” nanoemulsions for enhanced topical delivery of bakuchiol achieved in a sustainable manner: a novel eco-friendly approach to bioretinol. Int J Mol Sci 22:10091
Liu W, Hu M, Liu W (2008) Investigation of the carbopol gel of solid lipid nanoparticles for the transdermal iontophoretic delivery of triamcinolone acetonide acetate. Int J Pharm 364:135–141
Madhusudhan A, Reddy GB, Venkatesham M (2012) Design and evaluation of efavirenz loaded solid lipid nanoparticles to improve the oral bioavailability. Int J Pharm Pharm Sci 2:84–89
Mahajan M, Kaur M, Thakur S (2022) Solid lipid nanoparticles as carrier to increase local bioavailability of acitretin after topical administration in psoriasis treatment. J Pharm Innov 2022:1–18
Makarov SS (2001) NF-κB in rheumatoid arthritis: a pivotal regulator of inflammation, hyperplasia, and tissue destruction. Arthritis Res Ther 3:1–7
Malik A, Kanneganti TD (2018) Function and regulation of IL-1α in inflammatory diseases and cancer. Immunol Rev 281:124–137
Merclin N, Bramer T, Edsman K (2004) Iontophoretic delivery of 5-aminolevulinic acid and its methyl ester using a carbopol gel as vehicle. J Control Release 98:57–65
Moore KW, O’garra A, de Waal Malefyt R, (1993) Interleukin-10. Annu Rev Immunol 11:165–190
Müller RH, Mäder K, Gohla S (2000) Solid lipid nanoparticles (SLN) for controlled drug delivery–a review of the state of the art. Eur J Pharm Biopharm 50:161–177
Müller RH, Petersen RD, Hommoss A (2007) Nanostructured lipid carriers (NLC) in cosmetic dermal products. Adv Drug Deliv Rev 59:522–530
Nanda A, Nanda S, Nguyen TA (2020) Nanocosmetics: fundamentals, applications and toxicity. Micro and Nano Technologies 1–504.
Ohashi K, Parker JL, Ouchi N (2010) Adiponectin promotes macrophage polarization toward an anti-inflammatory phenotype. J Biol Chem 285:6153–6160
Palei NN, Mohanta BC, Das MK (2017) Lornoxicam loaded nanostructured lipid carriers for topical delivery: optimization, skin uptake and in vivo studies. J Drug Deliv Sci Technol 39:490–500
Parameswaran N, Patial S (2010) Tumor necrosis factor-α signaling in macrophages,.Critical Reviews™ in Eukaryotic Gene Expression 20:87–103.
Park EJ, Zhao YZK, YC, Sohn DH, (2005) Protective effect of (S)-bakuchiol from Psoralea corylifolia on rat liver injury in vitro and in vivo. Planta Med 71(06):508–513
Perera GK, Di Meglio P, Nestle FO (2012) Psoriasis. Annu Rev Pathol 7:385–422
Pradhan M, Singh D, Singh MR (2015) characterization and skin permeating potential of lipid based novel delivery system for topical treatment of psoriasis. Chem Phys Lipid 186:9–16
Pucek A, Tokarek B, Waglewska E (2020) Recent advances in the structural design of photosensitive agent formulations using “soft” colloidal nanocarriers. Pharmaceutics 12:587
Rajinikanth PS, Chellian J (2016) Development and evaluation of nanostructured lipid carrier-based hydrogel for topical delivery of 5-fluorouracil. Int J Nanomed 11:5067
Rajitha P, Shammika P, Aiswarya S (2019) Chaulmoogra oil based methotrexate loaded topical nanoemulsion for the treatment of psoriasis. J Drug Deliv Sci Technol 49:463–476
Robert C, Kupper TS (1999) Inflammatory skin diseases, T cells, and immune surveillance. N Engl J Med 341:1817–1828
Schottelius AJ, Mayo MW, Sartor RB (1999) Interleukin-10 signaling blocks inhibitor of κB kinase activity and nuclear factor κB DNA binding. J Biol Chem 274:31868–31874
Shamsuzzaman AS, Winnicki M, Wolk R (2004) Independent association between plasma leptin and C-reactive protein in healthy humans. Circulation 109:2181–2185
Singh S, Pradhan D, Puri P (2019) Genomic alterations driving psoriasis pathogenesis. Gene 683:61–71
Sinha AK (1972) Colorimetric assay of catalase. Anal Biochem 47:389–394
Szepietowski JC, Bielicka E, Nockowski P (2000) Increased interleukin-7 levels in the sera of psoriatic patients: lack of correlations with interleukin-6 levels and disease intensity: experimental dermatology Original article. Clin Exp Dermatol 25:643–647
Thomas L, Zakir F, Mirza MA (2017) Development of curcumin loaded chitosan polymer based nanoemulsion gel: in vitro, ex vivo evaluation and in vivo wound healing studies. Int J Biol Macromol 101:569–579
Tsuruta D (2009) NF-κB links keratinocytes and lymphocytes in the pathogenesis of psoriasis. Recent Pat Inflammation Allergy Drug Discovery 3:40–48
Wolf AM, Wolf D, Rumpold H (2004) Adiponectin induces the anti-inflammatory cytokines IL-10 and IL-1RA in human leukocytes. Biochem Biophys Res Commun 323:630–635
Yallapu MM, Nagesh PKB, Jaggi M (2015) Therapeutic applications of curcumin nanoformulations. AAPS J 17:1341–1356
Yamamoto Y, Gaynor RB (2001) Therapeutic potential of inhibition of the NF-κB pathway in the treatment of inflammation and cancer. J Clin Investig 107:135–142
Zhang S, Liu X, Mei L (2016) Epigallocatechin-3-gallate (EGCG) inhibits imiquimod-induced psoriasis-like inflammation of BALB/c mice. BMC Complement Altern Med 16:1–11