High-content assay to measure mitochondrial function and bacterial vacuole size in infected human primary macrophages.
Cell Biology
Cell-based Assays
High-throughput Screening
Immunology
Metabolism
Microbiology
Microscopy
Single Cell
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 Mar 2023
17 Mar 2023
Historique:
received:
21
10
2022
revised:
19
12
2022
accepted:
22
02
2023
entrez:
18
3
2023
pubmed:
19
3
2023
medline:
19
3
2023
Statut:
aheadofprint
Résumé
Regulation of bioenergetics and cell death are pivotal mitochondrial functions determining the responses of macrophages to infection. Here, we provide a protocol to investigate mitochondrial functions during infection of macrophages by intracellular bacteria. We describe steps for quantifying mitochondrial polarization, cell death, and bacterial infection in infected, living, human primary macrophages at the single-cell level. We also detail the use of the pathogen Legionella pneumophila as model. This protocol can be adapted to investigate mitochondrial functions in other settings. For complete details on the use and execution of this protocol, please refer to Escoll et al. (2021).
Identifiants
pubmed: 36933221
pii: S2666-1667(23)00133-8
doi: 10.1016/j.xpro.2023.102175
pmc: PMC10031535
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102175Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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