Ectopic expression of pigeonpea Orf147 gene imparts partial sterility in Cicer arietinum.

Cicer / genetics Ectopic Gene Expression Infertility Fabaceae Cajanus / genetics
CMS Cytoplasmic male sterility Heterosis Orf147 Transgenic chickpea

Journal

Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761

Informations de publication

Date de publication:
05 Jun 2023
Historique:
received: 03 01 2023
revised: 12 03 2023
accepted: 13 03 2023
medline: 1 5 2023
pubmed: 19 3 2023
entrez: 18 3 2023
Statut: ppublish

Résumé

Orf147, a cytotoxic peptide, has been found to cause cytoplasmic male sterility (CMS) in Cajanus cajanifolius (pigeonpea). In our study, Orf147 was introduced into self-pollinating Cicer arietinum (chickpea) using Agrobacterium-mediated transformation for induction of CMS. The stable integration and expression of the transgene has been assessed through PCR and qRT-PCR analysis. In addition, phenotypic sterility analysis has been performed, considering developmental parameters like flower development, pod formation and flower drop. Transgene inheritance analysis demonstrates that out of the five PCR positive events in the T

Identifiants

DOI: 10.1016/j.gene.2023.147372 PMID: 36933813
pubmed: 36933813
pii: S0378-1119(23)00213-5
doi: 10.1016/j.gene.2023.147372
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

147372

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Joorie Bhattacharya (J)

International Crops Research Institute for the Semi-arid Tropics (ICRISAT), Hyderabad, 502324 Telangana, India; Department of Genetics, Osmania University, Hyderabad, Telangana, India.

Dumbala Srinivas Reddy (D)

International Crops Research Institute for the Semi-arid Tropics (ICRISAT), Hyderabad, 502324 Telangana, India; ATGC Biotech Private Limited, ICCI Knowledge Park (IKP), Genome Valley, Hyderabad, 500078 Telangana, India.

Kalyani Prasad (K)

International Crops Research Institute for the Semi-arid Tropics (ICRISAT), Hyderabad, 502324 Telangana, India.

Rahul B Nitnavare (RB)

Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington LE12 5RD, United Kingdom; Plant Science Department, Rothamsted Research, Harpenden AL5 2JQ, United Kingdom.

Pooja Bhatnagar-Mathur (P)

International Maize and Wheat Improvement Center (CIMMYT), Carretera México-Veracruz 16 Km. 45, El Batán, Texcoco C.P. 56237, Mexico. Electronic address: p.b.mathur@cgiar.org.

Palakolanu Sudhakar Reddy (P)

International Crops Research Institute for the Semi-arid Tropics (ICRISAT), Hyderabad, 502324 Telangana, India. Electronic address: sudhakarreddy.palakolanu@icrisat.org.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Fabaceae › Cicer › Ectopic expression of pigeonpea Orf147 gene...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Expression génique ectopique › Ectopic expression of pigeonpea Orf147 gene...
questionsmedicales.fr › Maladies urogénitales › Maladies génitales › Infertilité › Ectopic expression of pigeonpea Orf147 gene...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Fabaceae › Ectopic expression of pigeonpea Orf147 gene...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Fabaceae › Cajanus › Ectopic expression of pigeonpea Orf147 gene...