Enzyme-Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells.
BONE FORMATION
BONE MARROW MESENCHYMAL STEM CELLS
BONE MARROW TRANSPLANTATION MODEL
OSTEOBLASTS
SYSTEM BIOLOGY—BONE INTERACTOR
Journal
JBMR plus
ISSN: 2473-4039
Titre abrégé: JBMR Plus
Pays: England
ID NLM: 101707013
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
received:
30
08
2022
revised:
27
12
2022
accepted:
09
01
2023
entrez:
20
3
2023
pubmed:
21
3
2023
medline:
21
3
2023
Statut:
epublish
Résumé
Mesenchymal stem cell (MSC) therapy is a promising approach to curing bone diseases and disorders. In treating genetic bone disorders, MSC therapy is local or systemic transplantation of isolated and in vitro proliferated MSC rather than bone marrow transplantation. Recent evidence showed that bone marrow MSC engraftment to bone regeneration has been controversial in animal and human studies. Here, our modified bone marrow transplantation (BMT) method solved this problem. Like routine BMT, our modified method involves three steps: (i) isolation of bone marrow cells from the donor, (ii) whole-body lethal irradiation to the recipient, and (iii) injection of isolated bone marrow cells into irradiated recipient mice via the tail vein. The significant modification is imported at the bone marrow isolation step. While the bone marrow cells are flushed out from the bone marrow with the medium in routine BMT, we applied the enzymes' (collagenase type 4 and dispase) integrated medium to wash out the bone marrow cells. Then, cells were incubated in enzyme integrated solution at 37°C for 10 minutes. This modification designated BMT as collagenase-integrated BMT (c-BMT). Notably, successful engraftment of bone marrow MSC to the new bone formation, such as osteoblasts and chondrocytes, occurs in c-BMT mice, whereas routine BMT mice do not recruit bone marrow MSC. Indeed, flow cytometry data showed that c-BMT includes a higher proportion of LepR
Identifiants
pubmed: 36936364
doi: 10.1002/jbm4.10722
pii: JBM410722
pmc: PMC10020919
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e10722Informations de copyright
© 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
Références
Front Cell Dev Biol. 2019 Nov 12;7:268
pubmed: 31799249
Blood. 2011 Jan 20;117(3):1053-60
pubmed: 21088133
Eur Rev Med Pharmacol Sci. 2016 Jul;20(13):2899-907
pubmed: 27424992
Blood Cells Mol Dis. 2013 Jan;50(1):41-9
pubmed: 22954476
Cells Tissues Organs. 2017;204(2):59-83
pubmed: 28647733
JCI Insight. 2022 Jan 11;7(1):
pubmed: 34874922
Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8932-7
pubmed: 12084934
Stem Cells. 2021 Nov;39(11):1457-1477
pubmed: 34224636
Mol Immunol. 2021 Mar;131:127-136
pubmed: 33441247
J Biomed Mater Res. 1996 Nov;32(3):333-40
pubmed: 8897137
Front Cell Dev Biol. 2022 Jan 18;9:798604
pubmed: 35118078
Cell Stem Cell. 2014 Aug 7;15(2):154-68
pubmed: 24953181
Stem Cells. 2007 Nov;25(11):2964-71
pubmed: 17656639
Biochem Biophys Res Commun. 2004 Apr 9;316(3):943-8
pubmed: 15033493
Mol Ther. 2013 Jun;21(6):1224-31
pubmed: 23587920
Eur Rev Med Pharmacol Sci. 2016 Mar;20(5):899-905
pubmed: 27010148
Cell Mol Neurobiol. 2018 Mar;38(2):467-477
pubmed: 28484859
Nature. 2013 Mar 14;495(7440):231-5
pubmed: 23434755
Blood. 2008 Apr 15;111(8):4386-91
pubmed: 18182575
Stem Cell Res Ther. 2020 Nov 23;11(1):492
pubmed: 33225992
Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11761-6
pubmed: 15282377
Blood. 2005 Jul 1;106(1):86-94
pubmed: 15769895
Stem Cells Int. 2016;2016:8906945
pubmed: 26880995
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15877-82
pubmed: 14673088
Methods Mol Biol. 2016;1416:123-46
pubmed: 27236669
Blood. 2001 Mar 1;97(5):1227-31
pubmed: 11222364
Adv Exp Med Biol. 2017;1041:5-18
pubmed: 29204826
Oncol Lett. 2019 Dec;18(6):6628-6638
pubmed: 31807176
Stem Cell Res Ther. 2014 Feb 07;5(1):22
pubmed: 24507665
Int J Med Sci. 2021 Feb 19;18(8):1824-1830
pubmed: 33746599
Blood. 2004 Nov 1;104(9):2728-35
pubmed: 15242873
Annu Rev Immunol. 2013;31:285-316
pubmed: 23298209
Int J Med Sci. 2018 May 22;15(8):748-757
pubmed: 30008583
Cancers (Basel). 2021 Dec 28;14(1):
pubmed: 35008304
Connect Tissue Res. 2017 Jan;58(1):2-14
pubmed: 27128146
Int J Stem Cells. 2020 Mar 30;13(1):1-12
pubmed: 31887851
Stem Cell Res Ther. 2019 Nov 20;10(1):331
pubmed: 31747966
Int J Med Sci. 2018 Sep 7;15(12):1406-1414
pubmed: 30275769
Transplantation. 2005 Jun 15;79(11):1607-14
pubmed: 15940052
Nat Cell Biol. 2017 Jun;19(6):677-688
pubmed: 28481328
Nature. 2012 Jan 25;481(7382):457-62
pubmed: 22281595
Exp Hematol. 2010 Jul;38(7):593-602
pubmed: 20417683
Front Immunol. 2020 Nov 25;11:585402
pubmed: 33324404
Mol Aspects Med. 2018 Aug;62:1-11
pubmed: 29410092