Erastin inhibits porcine epidemic diarrhea virus replication in Vero cells.
Ferroptosis
Ferrostatin-1
Vero cells
erastin
porcine epidemic diarrhea virus
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2023
2023
Historique:
received:
11
01
2023
accepted:
17
02
2023
entrez:
20
3
2023
pubmed:
21
3
2023
medline:
22
3
2023
Statut:
epublish
Résumé
Porcine epidemic diarrhea virus (PEDV), an intestinal pathogenic coronavirus, has caused significant economic losses to the swine industry worldwide. At present, there are several treatment methods, but there is still a lack of clinically effective targeted drugs, new antiviral mechanisms and drugs need to be explored. In this study, we established a model of erastin versus ferrostatin-1 treatment of Vero cells, and then detected virus proliferation and gene expression by RT-qPCR through PEDV infection experiments. We demonstrated for the first time that erastin significantly inhibited the replication of PEDV upon entry into cells; Vero treated with erastin significantly regulated the expression of three genes, Since
Sections du résumé
Background
Porcine epidemic diarrhea virus (PEDV), an intestinal pathogenic coronavirus, has caused significant economic losses to the swine industry worldwide. At present, there are several treatment methods, but there is still a lack of clinically effective targeted drugs, new antiviral mechanisms and drugs need to be explored.
Methods
In this study, we established a model of erastin versus ferrostatin-1 treatment of Vero cells, and then detected virus proliferation and gene expression by RT-qPCR through PEDV infection experiments.
Results
We demonstrated for the first time that erastin significantly inhibited the replication of PEDV upon entry into cells; Vero treated with erastin significantly regulated the expression of three genes,
Conclusions
Since
Identifiants
pubmed: 36936772
doi: 10.3389/fcimb.2023.1142173
pmc: PMC10015705
doi:
Substances chimiques
erastin
0
NF-E2-Related Factor 2
0
Piperazines
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1142173Informations de copyright
Copyright © 2023 Zhang, Li, Yang, Xiao, Dong, Lin, Liu and Shan.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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