In vivo measurement of the Young's modulus of the cell wall of single root hairs.
Bending
Cell wall
Mechanics
Root hair
Stiffness
Young’s modulus
Journal
Cell surface (Amsterdam, Netherlands)
ISSN: 2468-2330
Titre abrégé: Cell Surf
Pays: Netherlands
ID NLM: 101728565
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
20
12
2022
revised:
27
02
2023
accepted:
27
02
2023
entrez:
20
3
2023
pubmed:
21
3
2023
medline:
21
3
2023
Statut:
epublish
Résumé
Root hairs are cells from the root epidermis that grow as long tubular bulges perpendicular to the root. They can grow in a variety of mechanical or chemical environments. Their mechanical properties are mainly due to their stiff cell wall which also constitutes a physical barrier between the cell and its environment. Thus, it is essential to be able to quantify the cell wall mechanical properties and their adaptation to environmental cues. Here, we present a technique we developed to measure the Young's (elastic) modulus of the root hair cell wall. In essence, using custom-made glass microplates as cantilevers of calibrated stiffness, we are able to measure the force necessary to bend a single living root hair. From these experiments one can determine the stiffness and Young's modulus of the root hair cell wall.
Identifiants
pubmed: 36938503
doi: 10.1016/j.tcsw.2023.100104
pii: S2468-2330(23)00011-7
pmc: PMC10015226
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
100104Informations de copyright
© 2023 The Author(s).
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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