Recombinant Fusion Protein Vaccine Containing Clostridioides difficile FliC and FliD Protects Mice against C. difficile Infection.
Clostridioides difficile infection (CDI)
FliCD
chimeric protein vaccine
hyperimmune serum
Journal
Infection and immunity
ISSN: 1098-5522
Titre abrégé: Infect Immun
Pays: United States
ID NLM: 0246127
Informations de publication
Date de publication:
18 04 2023
18 04 2023
Historique:
medline:
20
4
2023
pubmed:
21
3
2023
entrez:
20
3
2023
Statut:
ppublish
Résumé
Bacterial flagella are involved in infection through their roles in host cell adhesion, cell invasion, auto-agglutination, colonization, the formation of biofilms, and the regulation and secretion of nonflagellar bacterial proteins that are involved in the virulence process. In this study, we constructed a fusion protein vaccine (FliCD) containing the Clostridioides difficile flagellar proteins FliC and FliD. The immunization of mice with FliCD induced potent IgG and IgA antibody responses against FliCD, protected mice against C. difficile infection (CDI), and decreased the C. difficile spore and toxin levels in the feces after infection. Additionally, the anti-FliCD serum inhibited the binding of C. difficile vegetative cells to HCT8 cells. These results suggest that FliCD may represent an effective vaccine candidate against CDI.
Identifiants
pubmed: 36939332
doi: 10.1128/iai.00169-22
pmc: PMC10112125
doi:
Substances chimiques
Recombinant Fusion Proteins
0
Bacterial Proteins
0
Bacterial Vaccines
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0016922Subventions
Organisme : NIAID NIH HHS
ID : R01 AI132711
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI149852
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI113470
Pays : United States
Organisme : NIH HHS
ID : K01-DK092352
Pays : United States
Références
Nature. 2010 Oct 7;467(7316):711-3
pubmed: 20844489
Biotechnol Bioeng. 2005 Jan 20;89(2):206-18
pubmed: 15593264
Microbiology (Reading). 2000 Apr;146 ( Pt 4):957-966
pubmed: 10784054
Mol Gen Genet. 1998 May;258(3):222-32
pubmed: 9645428
Infect Immun. 2011 Apr;79(4):1498-503
pubmed: 21245274
Infect Immun. 2011 Oct;79(10):4061-7
pubmed: 21788384
Microb Genom. 2020 Oct;6(10):
pubmed: 33030421
BMC Genomics. 2018 Jan 2;19(1):1
pubmed: 29291715
JAMA. 2015 Jan 27;313(4):398-408
pubmed: 25626036
J Struct Biol. 2001 Feb-Mar;133(2-3):246-53
pubmed: 11472095
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Comput Appl Biosci. 1993 Dec;9(6):745-56
pubmed: 8143162
J Bacteriol. 1984 Jan;157(1):100-8
pubmed: 6360991
J Bacteriol. 2010 Oct;192(19):4983-90
pubmed: 20675492
Clin Infect Dis. 2012 Aug;55 Suppl 2:S65-70
pubmed: 22752867
J Mol Biol. 2018 Jul 20;430(15):2237-2243
pubmed: 29258817
J Clin Microbiol. 2015 Oct;53(10):3141-7
pubmed: 26179308
Sci Rep. 2017 Jun 12;7(1):3256
pubmed: 28607468
Elife. 2016 Sep 24;5:
pubmed: 27664419
Nat Genet. 2006 Jul;38(7):779-86
pubmed: 16804543
JAMA. 2020 Apr 14;323(14):1403-1404
pubmed: 32150234
Curr Opin Genet Dev. 1992 Oct;2(5):756-67
pubmed: 1458024
JAMA Netw Open. 2020 Jan 3;3(1):e1917597
pubmed: 31913488
Microb Genom. 2020 Aug;6(8):
pubmed: 32726198
Gastroenterology. 2008 Dec;135(6):1984-92
pubmed: 18848941
Appl Environ Microbiol. 2012 Nov;78(21):7671-9
pubmed: 22923404
J Med Microbiol. 1993 Jun;38(6):434-41
pubmed: 8510136
Appl Environ Microbiol. 2012 Jul;78(13):4683-90
pubmed: 22522680
N Engl J Med. 2015 Jun 11;372(24):2368
pubmed: 26061851
Life Sci. 2013 Feb 27;92(3):211-7
pubmed: 23261530
J Clin Microbiol. 2000 Sep;38(9):3179-86
pubmed: 10970353
Infect Immun. 2001 Dec;69(12):7937-40
pubmed: 11705981
Genome Biol. 2009;10(9):R102
pubmed: 19781061
Clin Lab Med. 2017 Jun;37(2):341-369
pubmed: 28457354
Microbiol Spectr. 2022 Apr 27;10(2):e0178821
pubmed: 35315695
J AOAC Int. 2011 Mar-Apr;94(2):618-26
pubmed: 21563698
J Bacteriol. 2012 Apr;194(8):2125-6
pubmed: 22461557
Infect Immun. 2013 Jun;81(6):2190-6
pubmed: 23545305
Bioinformatics. 2009 May 1;25(9):1189-91
pubmed: 19151095
Hum Vaccin Immunother. 2015;11(9):2215-22
pubmed: 26036797
J Clin Microbiol. 2001 Mar;39(3):1178-83
pubmed: 11230454
Ann Intern Med. 2011 Dec 20;155(12):839-47
pubmed: 22184691
Gut Microbes. 2022 Jan-Dec;14(1):2038854
pubmed: 35192433
Emerg Microbes Infect. 2016 Feb 03;5:e8
pubmed: 26839147
Am J Physiol Lung Cell Mol Physiol. 2002 Apr;282(4):L751-6
pubmed: 11880301
Mol Gen Genet. 1994 Jun 15;243(6):605-12
pubmed: 8028576
JAMA Pediatr. 2013 Jun;167(6):592
pubmed: 23733223
Trends Microbiol. 2015 May;23(5):275-82
pubmed: 25659185
Int J Med Microbiol. 2017 Sep;307(6):311-320
pubmed: 28619474
Curr Protoc Bioinformatics. 2020 Dec;72(1):e108
pubmed: 33315308
Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7527-32
pubmed: 20368420
Ann Intern Med. 2018 Oct 2;169(7):ITC49-ITC64
pubmed: 30285209
Anaerobe. 2022 Feb;73:102476
pubmed: 34780914