Sodium Fluoride Exposure Leads to ATP Depletion and Altered RNA Decay in Escherichia coli under Anaerobic Conditions.
anaerobic growth
gene regulation
mRNA degradation
posttranscriptional regulation
sodium fluoride
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
20 Mar 2023
20 Mar 2023
Historique:
entrez:
20
3
2023
pubmed:
21
3
2023
medline:
21
3
2023
Statut:
aheadofprint
Résumé
Although fluoride-containing compounds are widely used to inhibit bacterial growth, the reprogramming of gene expression underlying cellular responses to fluoride, especially under anaerobic conditions, is still poorly understood. Here, we compare the genome-wide transcriptomic profiles of E. coli grown in the absence (control) or presence (20 and 70 mM) of sodium fluoride (NaF) under anaerobic conditions and assess the impact of fluoride-dependent ATP depletion on RNA turnover. Tiling array analysis revealed transcripts displaying altered abundance in response to NaF treatments. Quantile-based K-means clustering uncovered a subset of genes that were highly upregulated and then downregulated in response to increased and subsequently decreased fluoride concentrations, many of which (~40%) contained repetitive extragenic palindromic (REP) sequences. Northern blot analysis of some of these highly upregulated REP-containing transcripts (i.e.,
Identifiants
pubmed: 36939343
doi: 10.1128/spectrum.04158-22
pmc: PMC10100675
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0415822Références
Nature. 1995 Mar 16;374(6519):287-90
pubmed: 7533896
Science. 1997 Sep 5;277(5331):1453-62
pubmed: 9278503
Can J Microbiol. 1995 Nov;41(11):955-64
pubmed: 7497353
FEBS Lett. 1998 Nov 27;440(1-2):172-4
pubmed: 9862449
J Med Microbiol. 2016 Feb;65(2):147-159
pubmed: 26690690
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Cell. 1991 Jun 28;65(7):1233-42
pubmed: 1712252
Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):63-8
pubmed: 11134527
Biochim Biophys Acta. 1997 Jul 4;1320(3):217-34
pubmed: 9230919
Nat Rev Microbiol. 2013 Jan;11(1):45-57
pubmed: 23241849
Genomics. 2005 Jan;85(1):1-15
pubmed: 15607417
Mol Microbiol. 1994 Nov;14(4):731-41
pubmed: 7534370
FEMS Microbiol Rev. 2010 Sep;34(5):883-923
pubmed: 20659169
Annu Rev Microbiol. 2000;54:499-518
pubmed: 11018136
Sci Rep. 2020 Feb 26;10(1):3495
pubmed: 32103089
J Bacteriol. 2002 Oct;184(19):5502-7
pubmed: 12218039
Clin Microbiol Rev. 2019 Jan 30;32(2):
pubmed: 30700431
Genes Dev. 2021 Feb 1;35(3-4):286-299
pubmed: 33446571
Int J Mol Sci. 2022 Feb 25;23(5):
pubmed: 35269716
J Trop Pediatr. 2012 Dec;58(6):517-20
pubmed: 22718536
Philos Trans R Soc Lond B Biol Sci. 2018 Nov 5;373(1762):
pubmed: 30397102
Proc Natl Acad Sci U S A. 2017 Sep 19;114(38):E8025-E8034
pubmed: 28874523
Genes Dev. 1998 Sep 1;12(17):2770-81
pubmed: 9732274
Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11637-42
pubmed: 9751718
FEMS Microbiol Lett. 1994 Jun 15;119(3):283-8
pubmed: 8050711
Sci Total Environ. 2022 Feb 10;807(Pt 1):150601
pubmed: 34597567
Sci Rep. 2019 Feb 27;9(1):2934
pubmed: 30814571
Arch Microbiol. 2021 Aug;203(6):2747-2760
pubmed: 33791834
Chemosphere. 2014 Apr;100:190-3
pubmed: 24332731
Arch Toxicol. 2020 Apr;94(4):1051-1069
pubmed: 32152649
Biochem Cell Biol. 2015 Jun;93(3):236-40
pubmed: 25707819
Nature. 1996 May 9;381(6578):169-72
pubmed: 8610017
Biotechnol Bioeng. 1996 Nov 5;52(3):364-72
pubmed: 18629906
Front Immunol. 2018 Oct 09;9:2318
pubmed: 30356663
Caries Res. 1972;6(2):93-102
pubmed: 4502285
J Bacteriol. 1981 Apr;146(1):128-32
pubmed: 6163761
J Clin Invest. 1971 Aug;50(8):1731-7
pubmed: 4329003
Microbiol Spectr. 2015 Jun;3(3):
pubmed: 26185089
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613
pubmed: 30476243
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2758-63
pubmed: 14981237
Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):3865-9
pubmed: 8632981
Curr Opin Microbiol. 1999 Apr;2(2):181-7
pubmed: 10322162
J Bacteriol. 2006 Oct;188(20):7165-75
pubmed: 17015655
Front Microbiol. 2021 Aug 16;12:705326
pubmed: 34484145
J Oral Microbiol. 2017 Jul 6;9(1):1344509
pubmed: 28748043
Biochemistry. 2006 Jan 24;45(3):793-800
pubmed: 16411755