Two-Dimensional Supramolecular Polymerization of DNA Amphiphiles is Driven by Sequence-Dependent DNA-Chromophore Interactions.

DNA Molecular Dynamics Simulations Nanosheets Self-Assembly Sequence-Controlled Polymers

Journal

Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543

Informations de publication

Date de publication:
12 06 2023
Historique:
received: 05 12 2022
pmc-release: 12 06 2024
medline: 5 6 2023
pubmed: 21 3 2023
entrez: 20 3 2023
Statut: ppublish

Résumé

Two-dimensional (2D) assemblies of water-soluble block copolymers have been limited by a dearth of systematic studies that relate polymer structure to pathway mechanism and supramolecular morphology. Here, we employ sequence-defined triblock DNA amphiphiles for the supramolecular polymerization of free-standing DNA nanosheets in water. Our systematic modulation of amphiphile sequence shows the alkyl chain core forming a cell membrane-like structure and the distal π-stacking chromophore block folding back to interact with the hydrophilic DNA block on the nanosheet surface. This interaction is crucial to sheet formation, marked by a chiral "signature", and sensitive to DNA sequence, where nanosheets form with a mixed sequence, but not with a homogeneous poly(thymine) sequence. This work opens the possibility of forming well-ordered, bilayer-like assemblies using a single DNA amphiphile for applications in cell sensing, nucleic acid therapeutic delivery and enzyme arrays.

Identifiants

pubmed: 36939824
doi: 10.1002/anie.202217814
pmc: PMC10239398
mid: NIHMS1888468
doi:

Substances chimiques

Peptides 0
Polymers 0
DNA 9007-49-2
Water 059QF0KO0R

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202217814

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM129431
Pays : United States

Informations de copyright

© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.

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Auteurs

Muhammad Ghufran Rafique (M)

Department of Chemistry, McGill University, 801 Sherbrooke St W, H3A 0B8, Montréal, QC, Canada.

Jacob M Remington (JM)

Department of Chemistry, The University of Vermont, 05405, Burlington, VT, USA.

Finley Clark (F)

Department of Chemistry, The University of Vermont, 05405, Burlington, VT, USA.

Haochen Bai (H)

Department of Chemistry, McGill University, 801 Sherbrooke St W, H3A 0B8, Montréal, QC, Canada.

Violeta Toader (V)

Department of Chemistry, McGill University, 801 Sherbrooke St W, H3A 0B8, Montréal, QC, Canada.

Dmytro F Perepichka (DF)

Department of Chemistry, McGill University, 801 Sherbrooke St W, H3A 0B8, Montréal, QC, Canada.

Jianing Li (J)

Department of Chemistry, The University of Vermont, 05405, Burlington, VT, USA.
Current address: Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN, 47907, USA.

Hanadi F Sleiman (HF)

Department of Chemistry, McGill University, 801 Sherbrooke St W, H3A 0B8, Montréal, QC, Canada.

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Classifications MeSH